一种用于流式细胞分选仪的实时延迟监测器。

A real-time delay monitor for flow-system cell sorters.

作者信息

Martin J C, McLaughlin S R, Hiebert R D

出版信息

J Histochem Cytochem. 1979 Jan;27(1):277-9. doi: 10.1177/27.1.374586.

DOI:10.1177/27.1.374586

Abstract

For optimum performance in cell sorting, it is critical to assure proper timing in the charging of droplets to be deflected. A method for determining the transiet delay time in cell sorters has been devised and applied to daily operation in the Los Alamos sorter systems. This delay monitor relies on detection of either scattered or absorbed light from cells in the fluid stream near the point of droplet breakoff.

摘要

为了在细胞分选过程中实现最佳性能，确保待偏转液滴充电的正确时间至关重要。一种确定细胞分选仪中瞬态延迟时间的方法已经被设计出来，并应用于洛斯阿拉莫斯分选仪系统的日常操作中。这种延迟监测器依靠检测液滴断裂点附近流体流中细胞的散射光或吸收光。