Merrill J T, Dean P N, Gray J W
J Histochem Cytochem. 1979 Jan;27(1):280-3. doi: 10.1177/27.1.374587.
We have investigated the accuracy with which droplets containing cells can be sorted individually onto known and thus relocatable positions. The presence and random arrival of cells and particles in the sorter jet disturbs the orderly production and deflection of droplets, causing a dispersion of sorted droplet trajectories. The magnitude of this dispersion is a function of the phase relationship between the arrival of a cell at the end of the jet and the droplet formation. Using a modified Becton Dickinson Fluorescence-Activated Cell Sorter, we selected for sorting only those droplets that formed with a cell near the center of the droplet. We used this technique to sort Lewis lung tumor cells. The dispersion of droplet positions was reduced from over 3% to about 1% of an average deflection of typically 15 mm for a nozzle with a 50-micron diameter orifice. Sorting onto a surface such as magnetic tape or a microscope slide introduces another uncertainty in position because the cell may be located anywhere within the wetted radius of the droplet on the slide. Sorting onto less-wettable surfaces reduces the wetted radius and thus the variation in cell position.
我们已经研究了含有细胞的液滴能够被逐个分选到已知且因此可重新定位的位置上的准确性。细胞和颗粒在分选器射流中的存在及随机到达会干扰液滴的有序产生和偏转，导致分选后的液滴轨迹分散。这种分散的程度是细胞在射流末端到达与液滴形成之间相位关系的函数。使用改进后的贝克顿·迪金森荧光激活细胞分选仪，我们选择仅对那些在液滴中心附近有细胞形成的液滴进行分选。我们使用这种技术对刘易斯肺癌细胞进行分选。对于直径为50微米孔口的喷嘴，液滴位置的分散从超过平均偏转量的3%降低到了约1%，典型的平均偏转量为15毫米。分选到诸如磁带或显微镜载玻片等表面上会在位置上引入另一个不确定性，因为细胞可能位于载玻片上液滴的湿润半径内的任何位置。分选到润湿性较差的表面上会减小湿润半径，从而减少细胞位置的变化。
