Stovel R T, Sweet R G
J Histochem Cytochem. 1979 Jan;27(1):284-8. doi: 10.1177/27.1.374588.
Current cell sorting machines do not preserve the individual identity of processed cells; after analysis, the cells are assigned to a subpopulation where they are pooled with other similar cells. This paper reports progress on a system that sorts cells individually to precise locations on a microscope slide and preserves them for further observation with a light microscope while recording flow measurement data for each cell. Various electronic and mechanical modifications to an existing sorting machine are described that increase drop placement accuracy and permit individual cell sorting.
当前的细胞分选机无法保留被处理细胞的个体身份;分析之后,细胞被归入一个亚群,与其他相似细胞汇集在一起。本文报告了一个系统所取得的进展,该系统可将细胞逐个分选到显微镜载玻片上的精确位置,并将其保存以便用光显微镜进一步观察,同时记录每个细胞的流量测量数据。文中描述了对现有分选机进行的各种电子和机械改进,这些改进提高了液滴放置的准确性,并允许进行单个细胞分选。