Shimada M, Shimono R, Imahaysi T, Ozaki S H, Yamaguchi K, Niizeki S
J Histochem Cytochem. 1986 Oct;34(10):1281-92. doi: 10.1177/34.10.3745908.
The distribution and fate of 35S from [35S]-cysteine and 14C from [1-14C]-cystine, both precursors to taurine in 17-day pregnant mice and (1-day-old) neonates were investigated by whole-body autoradiography following IV injection for the pregnant mice and IP injection for the neonates. Survival intervals were 30 min, 3 hr. In the dam, 35S and 14C were both highly incorporated into the pancreas. As very low uptake of [35S]-taurine in the pancreas was found in a previous study, it is reasonable to suggest that most radioactivity found in the pancreas represents cysteine. In maternal brain, brown fat, and myocardium, optical density and relative ratio (ODs of 35S and 14C in each tissue and organ/ODs of 35S and 14C in the blood) of 35S were significantly higher than those of 14C, assuming that most of 35S might be present as taurine in these regions. In the developing brain, 35S was found mainly in the differentiating neurons of the cortical plate, including the primordial hippocampal cell layer, and of the cerebellar cortical plate. Relative ratios of 35S in these cerebral regions were significantly higher than those of 14C, suggesting that most of 35S represents taurine.
通过对怀孕小鼠静脉注射以及对新生小鼠腹腔注射后进行全身放射自显影，研究了[35S]-半胱氨酸中的35S和[1-14C]-胱氨酸中的14C（这两种都是17天龄怀孕小鼠和1日龄新生小鼠中牛磺酸的前体）的分布和去向。存活时间间隔为30分钟、3小时。在母鼠中，35S和14C都高度掺入胰腺。由于在先前的一项研究中发现胰腺对[35S]-牛磺酸的摄取非常低，因此有理由认为在胰腺中发现的大部分放射性代表半胱氨酸。在母体大脑、棕色脂肪和心肌中，35S的光密度和相对比率（每个组织和器官中35S和14C的光密度/血液中35S和14C的光密度）显著高于14C，假设在这些区域中大部分35S可能以牛磺酸的形式存在。在发育中的大脑中，35S主要存在于皮质板的分化神经元中，包括原始海马细胞层和小脑皮质板。这些脑区中35S的相对比率显著高于14C，表明大部分35S代表牛磺酸。
