35S-牛磺酸在新生和成年大鼠体内的分布：一项全身放射自显影研究。

Distribution of 35S-taurine in rat neonates and adults. A whole-body autoradiographic study.

作者信息

Shimada M, Shimono R, Watanabe M, Imahayashi T, Ozaki H S, Kihara T, Yamaguchi K, Niizeki S

出版信息

Histochemistry. 1984;80(3):225-30. doi: 10.1007/BF00495770.

Abstract

The distribution of 35S-taurine in rat neonates and adults was investigated by wholy -body autoradiography. The neonates (4-day-old) were frozen in dry-ice hexane at 30 min, 1, 3 and 6 h after an intraperitoneal injection of 35S-taurine, whereas survival intervals for adult rats were 1 and 3 h. Whole-sagittal sections of the frozen rat, obtained by using a cryostat microtome were dried in situ and autoradiographed. In rat neonates and adults, 35S-taurine was mainly accumulated in the renal cortex, urine, feces, liver, eye (lens, vitreous fluid, retina), hypophysis, thymus, adrenal glands, nasal mucous membrane, salivary glands, gastric mucosa, small and large intestinal mucosa, choroid plexus, myocardium and sebaceous glands. In the rat neonate, such regions as the olfactory bulb, cerebrum, and cerebellum showed relatively high optical density.

摘要

采用全身放射自显影法研究了35S-牛磺酸在新生大鼠和成年大鼠体内的分布情况。新生大鼠（4日龄）在腹腔注射35S-牛磺酸后30分钟、1小时、3小时和6小时，置于干冰己烷中冷冻，而成年大鼠的存活时间间隔为1小时和3小时。使用低温切片机获取冷冻大鼠的全矢状切片，原位干燥后进行放射自显影。在新生大鼠和成年大鼠中，35S-牛磺酸主要蓄积于肾皮质、尿液、粪便、肝脏、眼睛（晶状体、玻璃体、视网膜）、垂体、胸腺、肾上腺、鼻黏膜、唾液腺、胃黏膜、小肠和大肠黏膜、脉络丛、心肌和皮脂腺。在新生大鼠中，嗅球、大脑和小脑等区域显示出相对较高的光密度。