引发酶-聚合酶合成引物的机制。

Mechanism of primer synthesis by Primase-Polymerases.

作者信息

Zabrady Katerina, Li Arthur W H, Doherty Aidan J

机构信息

Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton BN1 9RQ, UK. Electronic address: https://twitter.com/@KZabrady.

Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton BN1 9RQ, UK.

出版信息

Curr Opin Struct Biol. 2023 Oct;82:102652. doi: 10.1016/j.sbi.2023.102652. Epub 2023 Jul 15.

DOI:10.1016/j.sbi.2023.102652

PMID:37459807

Abstract

Members of the primase-polymerase (Prim-Pol) superfamily are found in all domains of life and play diverse roles in genome stability, including primer synthesis during DNA replication, lesion repair and damage tolerance. This review focuses primarily on Prim-Pol members capable of de novo primer synthesis that have experimentally derived structural models available. We discuss the mechanism of DNA primer synthesis initiation by Prim-Pol catalytic domains, based on recent structural and functional studies. We also describe a general model for primer initiation that also includes the ancillary domains/subunits, which stimulate the initiation of primer synthesis.

摘要

引发酶-聚合酶（Prim-Pol）超家族成员存在于生命的所有领域，并在基因组稳定性中发挥多种作用，包括DNA复制过程中的引物合成、损伤修复和损伤耐受。本综述主要关注能够进行从头引物合成且有实验得出的结构模型的Prim-Pol成员。基于最近的结构和功能研究，我们讨论了Prim-Pol催化结构域引发DNA引物合成的机制。我们还描述了一个引物起始的通用模型，该模型也包括辅助结构域/亚基，其可刺激引物合成的起始。