Nakabayashi Y, Yasuda M, Murae M, Takahashi S, Nakata H, Fujiya S, Dozono H, Ochiai K, Inui H, Yoshioka M
Nihon Sanka Fujinka Gakkai Zasshi. 1986 Jul;38(7):1087-94.
While the treatment of ovarian cancer has made remarkable progress in recent years, the chemotherapy of ovarian germ cell tumor has not as yet been fully established and remains to be improved. We investigated the effectiveness of a single-agent therapy with Vincristine (VCR), Actinomycin D (ACD), and Cyclophosphamide (CPM) against a human undifferentiated dysgerminoma's cell line (JOHYL-1) with the following results. The ratio of the number of viable cells to the total cell population treated for JOHYL-1 cells of ascitic type was 70% with VCR, 58% with CPM and 25% with ACD; hence the efficacy on this malignant cell line was the greatest with ACD followed by VCR and CPM in that descending order. Notably with CPM, a striking antitumor effect was observed from 48 to 24 hours after treatment. The L.I. attained a peak at 24, 18 and 30 hours, respectively, after treatment with VCR, ACD and CPM. The M.I. was as high as a little more than 6% at 36 hours after treatment with VCR. There also was noted a shift in the cell cycle with any of the test drugs. Morphological changes observed in the cell were most obvious with ACD followed in order by VCR and CPM. LDH & isozyme pattern: In these respects there were no striking differences among the three drugs, even though the total enzyme activity was somewhat higher with VCR.(ABSTRACT TRUNCATED AT 250 WORDS)
尽管近年来卵巢癌的治疗取得了显著进展，但卵巢生殖细胞肿瘤的化疗尚未完全确立，仍有待改进。我们研究了长春新碱（VCR）、放线菌素D（ACD）和环磷酰胺（CPM）单药治疗人未分化无性细胞瘤细胞系（JOHYL-1）的有效性，结果如下。腹水型JOHYL-1细胞经处理后，活细胞数与总细胞数的比例，VCR处理组为70%，CPM处理组为58%，ACD处理组为25%；因此，对该恶性细胞系的疗效以ACD最大，其次是VCR和CPM，呈递减顺序。值得注意的是，CPM在治疗后48至24小时观察到显著的抗肿瘤作用。VCR、ACD和CPM处理后，L.I.分别在24、18和30小时达到峰值。VCR处理后36小时，M.I.高达6%多一点。使用任何一种受试药物后，细胞周期均发生了变化。细胞中观察到的形态学变化以ACD最为明显，其次是VCR和CPM。乳酸脱氢酶（LDH）及同工酶模式：在这些方面，三种药物之间没有显著差异，尽管VCR处理组的总酶活性略高。（摘要截短于250字）
