Laboratory of Veterinary Infectious Disease, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
J Vet Med Sci. 2023 Sep 1;85(9):907-911. doi: 10.1292/jvms.23-0185. Epub 2023 Jul 17.
Chlamydia-related bacteria of the Chlamydiales order have recently been described as emerging pathogens that cause pneumonia and abortion in animals and humans. We investigated the presence of Chlamydiales using real-time polymerase chain reaction (PCR) by targeting the 16S rRNA gene of a broad range of Chlamydiales in 827 fecal samples from pet birds kept in individual homes in Japan. Of the 827 samples, 493 (59.6%) tested positive for the Chlamydiales 16S rRNA gene in the real-time PCR assay. We determined the nucleic acid sequences of PCR products from 17 Chlamydiales strains. A homology search and phylogenetic analysis using these sequences confirmed that the detected Chlamydiales included C. pecorum and a broad range of Chlamydia-related bacteria. To the best of our knowledge, this is the first study to detect a wide range of Chlamydia-related bacteria in birds.
最近，被描述为新兴病原体的衣原体目相关细菌已被发现可引起动物和人类肺炎和流产。我们使用针对衣原体目广泛范围的 16S rRNA 基因的实时聚合酶链反应（PCR），对来自日本个体家庭饲养的宠物鸟的 827 份粪便样本进行了研究。在 827 个样本中，493 个（59.6%）在实时 PCR 检测中检测到衣原体目 16S rRNA 基因呈阳性。我们从 17 株衣原体目中确定了 PCR 产物的核酸序列。使用这些序列进行的同源性搜索和系统发育分析证实，检测到的衣原体目包括鹦鹉热衣原体和广泛范围的衣原体相关细菌。据我们所知，这是首次在鸟类中检测到广泛范围的衣原体相关细菌。
