Szymańska-Czerwińska Monika, Mitura Agata, Zaręba Kinga, Schnee Christiane, Koncicki Andrzej, Niemczuk Krzysztof
Department of Cattle and Sheep Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland.
Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut, 07 743 Jena, Germany.
J Vet Res. 2017 Dec 27;61(4):411-419. doi: 10.1515/jvetres-2017-0072. eCollection 2017 Dec.
The study was conducted to investigate the prevalence and genetic diversity of spp. in poultry in Poland and estimate possible transmission to humans.
Molecular diagnostic methods followed by sequencing and strain isolation were used on cloacal/faecal swabs collected from 182 apparently healthy poultry flocks including chickens, turkeys, ducks, and geese. Serum samples obtained from people exposed (study group) and non-exposed (control group) to birds were tested by complement fixation test to acquire data on spp. antibody level.
Overall, 15.9% of the tested flocks were -positive and three spp. were identified. Predominant chlamydial agent found was occurring in 65.5% of all positive poultry flocks and in 73.0% of positive chicken flocks. The sequences from four chicken flocks were assigned to , whereas was confirmed in one duck and one goose flock. The analysis of A variable domains revealed at least nine genetic variants of . Chlamydial antibodies were detected in 19.2% of human serum samples in the study group in comparison with 10.8% in the controls.
The obtained results confirm that chlamydiae are common among chicken flocks in Poland with as a dominant species. Moreover, the presence of in chickens is reported here for the first time. Further investigation should focus on possible zoonotic transmission of and as well as potential pathogenic effects on birds' health and poultry production.
本研究旨在调查波兰家禽中 spp. 的流行情况和遗传多样性，并评估其向人类传播的可能性。
对从182个看似健康的家禽群（包括鸡、火鸡、鸭和鹅）采集的泄殖腔/粪便拭子采用分子诊断方法，随后进行测序和菌株分离。通过补体结合试验对接触鸟类（研究组）和未接触鸟类（对照组）的人群的血清样本进行检测，以获取有关 spp. 抗体水平的数据。
总体而言，15.9% 的检测禽群为 -阳性，并鉴定出三种 spp.。发现的主要衣原体病原体是 ，在所有阳性家禽群中占65.5%，在阳性鸡群中占73.0%。来自四个鸡群的序列被归类为 ，而在一个鸭群和一个鹅群中证实存在 。对A可变区的分析揭示了至少九种 的遗传变体。研究组中19.2% 的人类血清样本检测到衣原体抗体，而对照组为10.8%。
所得结果证实衣原体在波兰鸡群中很常见， 为优势种。此外，本文首次报道了鸡群中存在 。进一步的研究应集中在 和 可能的人畜共患病传播以及对鸟类健康和家禽生产潜在的致病影响上。
