Laboratory of Veterinary Infectious Disease, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
J Vet Med Sci. 2022 Jun 17;84(6):817-823. doi: 10.1292/jvms.21-0600. Epub 2022 Apr 28.
The members of family Chlamydiaceae have a broad host range and cause many kinds of diseases in humans and animals. Several cases of Chlamydiaceae being detected in atypical hosts have been reported recently. Consequently, cross-species monitoring of Chlamydia in wildlife and livestock is pertinent for public health, animal hygiene and wildlife conservation. In this study, we conducted molecular surveillance of Chlamydia in wild birds and livestock around a small village in the foothills of Mt. Afadjato, Ghana where direct contact between wildlife and livestock occurs. Among 29 captured wild birds and 63 livestock, 5 sheep, 30 goats and 28 chickens, the positive ratios of Chlamydia were 24.1%, 40.0%, 43.3% and 26.9%, respectively. Chlamydia pecorum was detected in wild birds, goats, sheep and chickens. On the basis of the variable domain 2 region of ompA, several samples from different hosts showed identical sequences and were phylogenetically located to the same clusters. In addition, using ompA, C. psittaci, C. abortus and C. gallinacea were also detected in this small habitat. Further genetic and pathogenic analyses of the chlamydial distribution in this area, which represents the interface of wild and domestic animal interactions, may improve our knowledge of their transmission among different hosts.
科衣原体家族的成员宿主范围广泛，可引起人类和动物的多种疾病。最近有报道称，在非典型宿主中检测到几种衣原体。因此，对野生动物和家畜中的衣原体进行跨物种监测与公共卫生、动物卫生和野生动物保护息息相关。在这项研究中，我们对加纳阿法贾托山脚下一个小村庄周围的野生鸟类和家畜进行了衣原体的分子监测，那里野生动物和家畜直接接触。在 29 只捕获的野生鸟类和 63 只家畜（5 只绵羊、30 只山羊和 28 只鸡）中，衣原体的阳性率分别为 24.1%、40.0%、43.3%和 26.9%。在野生鸟类、山羊、绵羊和鸡中检测到了绵羊衣原体。基于 omaP 基因的可变区 2 区，来自不同宿主的几个样本显示出相同的序列，并在系统发育上位于相同的聚类中。此外，在这个小栖息地中，还利用 omaP 基因检测到了鹦鹉热衣原体、流产衣原体和鸡衣原体。进一步对该地区衣原体分布的遗传和致病性进行分析，该地区代表了野生动物和家畜相互作用的界面，可能有助于提高我们对不同宿主间传播的认识。
