CCR4-NOT 复合物的 NOT10:11 模块的结构与组装。
Structure and assembly of the NOT10:11 module of the CCR4-NOT complex.
机构信息
RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA.
Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, Germany.
出版信息
Commun Biol. 2023 Jul 17;6(1):739. doi: 10.1038/s42003-023-05122-4.
NOT1, NOT10, and NOT11 form a conserved module in the CCR4-NOT complex, critical for post-transcriptional regulation in eukaryotes, but how this module contributes to the functions of the CCR4-NOT remains poorly understood. Here, we present cryo-EM structures of human and chicken NOT1:NOT10:NOT11 ternary complexes to sub-3 Å resolution, revealing an evolutionarily conserved, flexible structure. Through biochemical dissection studies, which include the Drosophila orthologs, we show that the module assembly is hierarchical, with NOT11 binding to NOT10, which then organizes it for binding to NOT1. A short proline-rich motif in NOT11 stabilizes the entire module assembly.
NOT1、NOT10 和 NOT11 在 CCR4-NOT 复合物中形成一个保守模块，对于真核生物的转录后调控至关重要，但该模块如何影响 CCR4-NOT 的功能仍知之甚少。在这里，我们呈现了人类和鸡 NOT1：NOT10：NOT11 三元复合物的低温电镜结构，分辨率达到亚 3Å，揭示了一个进化上保守的、灵活的结构。通过包括果蝇同源物在内的生化剖析研究，我们表明模块组装是分层次的，NOT11 首先与 NOT10 结合，然后将其组织起来与 NOT1 结合。NOT11 中的一个短脯氨酸丰富的基序稳定了整个模块组装。
Zotero 插件