Department of Orthopedics, The Second Affiliated Hospital, Shantou University Medical College, Shantou, 515000, Guangdong, China.
Department of Shantou Central Hospital, Shantou, 515000, Guangdong, China.
In Vitro Cell Dev Biol Anim. 2023 Jun;59(6):420-430. doi: 10.1007/s11626-023-00783-1. Epub 2023 Jul 17.
Brevilin A (BA) is the primary component of Centipeda minima, which is widely used in Chinese traditional medicine. The anti-inflammatory and anti-tumor properties of BA have been established; however, its function in bone metabolism is not well understood. This study revealed that concentrations of BA below 1.0 µM did not inhibit the proliferation of bone marrow macrophages but did impede the differentiation and bone resorption activity of osteoclasts. Furthermore, BA suppressed the expression of osteoclast-specific genes Mmp9, Acp5, Dc-stamp, Ctsk, and Atp6v0d2. In addition, mTOR, ERK, and NFATc1 activation in bone marrow macrophages were suppressed by BA. As a whole, BA blocks the mTOR and ERK signaling pathways, which is responsible for the development and activity of osteoclasts, and the resorption of bone.
Brevilin A (BA) 是小飞扬草的主要成分，小飞扬草在中药中被广泛应用。BA 具有抗炎和抗肿瘤特性，但其在骨代谢中的作用尚不清楚。本研究表明，浓度低于 1.0µM 的 BA 不会抑制骨髓巨噬细胞的增殖，但会阻碍破骨细胞的分化和骨吸收活性。此外，BA 抑制破骨细胞特异性基因 Mmp9、Acp5、Dc-stamp、Ctsk 和 Atp6v0d2 的表达。此外，BA 还抑制了骨髓巨噬细胞中 mTOR、ERK 和 NFATc1 的激活。总的来说，BA 阻断了负责破骨细胞的发育和活性以及骨吸收的 mTOR 和 ERK 信号通路。
