John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
Harper Adams University, Newport, Shropshire, TF10 8NB, UK.
BMC Plant Biol. 2023 Jul 18;23(1):363. doi: 10.1186/s12870-023-04369-7.
Durum wheat (Triticum turgidum subsp. durum) is widely grown for pasta production, and more recently, is gaining additional interest due to its resilience to warm, dry climates and its use as an experimental model for wheat research. Like in bread wheat, the starch and protein accumulated in the endosperm during grain development are the primary contributors to the calorific value of durum grains.
To enable further research into endosperm development and storage reserve synthesis, we generated a high-quality transcriptomics dataset from developing endosperms of variety Kronos, to complement the extensive mutant resources available for this variety. Endosperms were dissected from grains harvested at eight timepoints during grain development (6 to 30 days post anthesis (dpa)), then RNA sequencing was used to profile the transcriptome at each stage. The largest changes in gene expression profile were observed between the earlier timepoints, prior to 15 dpa. We detected a total of 29,925 genes that were significantly differentially expressed between at least two timepoints, and clustering analysis revealed nine distinct expression patterns. We demonstrate the potential of our dataset to provide new insights into key processes that occur during endosperm development, using starch metabolism as an example.
We provide a valuable resource for studying endosperm development in this increasingly important crop species.
硬质小麦（Triticum turgidum subsp. durum）广泛用于面食生产，最近由于其对温暖干燥气候的适应性以及作为小麦研究实验模型的用途而受到更多关注。与面包小麦一样，在谷物发育过程中，胚乳中积累的淀粉和蛋白质是硬质谷物热量值的主要贡献者。
为了能够进一步研究胚乳发育和储存物质的合成，我们从品种 Kronos 的发育胚乳中生成了一个高质量的转录组数据集，以补充该品种可用的广泛的突变体资源。从小麦授粉后 6 到 30 天（6 到 30 dpa）的八个时间点收获的谷物中分离胚乳，然后使用 RNA 测序在每个阶段对转录组进行分析。在 15 dpa 之前的早期时间点观察到基因表达谱的最大变化。我们总共检测到 29925 个基因在至少两个时间点之间存在显著差异表达，聚类分析显示出九个不同的表达模式。我们以淀粉代谢为例，展示了我们的数据集在研究这个日益重要的作物物种胚乳发育方面提供新见解的潜力。
我们为研究这个日益重要的作物物种的胚乳发育提供了一个有价值的资源。
