优先表达的胚乳基因揭示了小麦胚乳在灌浆过程中的独特活动。

Preferentially expressed endosperm genes reveal unique activities in wheat endosperm during grain filling.

机构信息

Institute of Nuclear and Biological Technologies/Xinjiang Key Laboratory of Crop Biotechnology/Key Laboratory of Oasis-Desert Crop Physiology Ecology and Cultivation of Ministry of Agricultural and Rural Affairs/Crop Chemical Regulation Engineering Technology Research Center in Xinjiang, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, China.

State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China.

出版信息

BMC Genomics. 2024 Aug 22;25(1):795. doi: 10.1186/s12864-024-10713-4.

DOI:10.1186/s12864-024-10713-4

PMID:39174916

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11340063/

Abstract

BACKGROUND

Bread wheat (Triticum aestivum L.) endosperm contains starch and proteins, which determine the final yield, quality, and nutritional value of wheat grain. The preferentially expressed endosperm genes can precisely provide targets in the endosperm for improving wheat grain quality and nutrition using modern bioengineering technologies. However, the genes specifically expressed in developing endosperms remain largely unknown.

RESULTS

In this study, 315 preferentially expressed endosperm genes (PEEGs) in the spring wheat landrace, Chinese Spring, were screened using data obtained from an open bioinformatics database, which reveals a unique grain reserve deposition process and special signal transduction in a developing wheat endosperm. Furthermore, transcription and accumulation of storage proteins in the wheat cultivar, XC26 were evaluated. The results revealed that 315 PEEG plays a critical role in storage protein fragment deposition and is a potential candidate for modifying grain quality and nutrition.

CONCLUSION

These results provide new insights into endosperm development and candidate genes and promoters for improving wheat grain quality through genetic engineering and plant breeding techniques.

摘要

背景

小麦胚乳含有淀粉和蛋白质，决定了小麦籽粒的最终产量、品质和营养价值。优先表达的胚乳基因可以利用现代生物工程技术，在胚乳中精确地为提高小麦籽粒品质和营养价值提供目标。然而，发育中的胚乳中特异性表达的基因在很大程度上仍然未知。

结果

本研究利用公开的生物信息学数据库获得的数据，筛选出春小麦地方品种中国春中 315 个优先表达的胚乳基因（PEEGs），揭示了发育中的小麦胚乳中独特的谷物贮藏沉积过程和特殊的信号转导。此外，还评估了小麦品种 XC26 中贮藏蛋白的转录和积累。结果表明，315 个 PEEG 在贮藏蛋白片段沉积中起着关键作用，是改良籽粒品质和营养的潜在候选基因。