一种反式作用机制抑制小鼠杂交胸腺瘤细胞系中主要移植抗原的表达。
A trans-acting mechanism represses the expression of the major transplantation antigens in mouse hybrid thymoma cell lines.
作者信息
Baldacci P, Transy C, Cochet M, Penit C, Israel A, Kourilsky P
出版信息
J Exp Med. 1986 Sep 1;164(3):677-94. doi: 10.1084/jem.164.3.677.
Abstract
We have fused an H-2- thymoma (BM5R.9) with an H-2+ thymoma (BW5147) and have found that many of the resulting hybrids exhibit an H-2- phenotype. In several hybrids that were analyzed in detail, this phenotype is related to the absence of steady-state H-2 mRNA and shows some instability, possibly related to the loss of chromosomes in segregants. We conclude from our studies that BM5R.9 cells display a trans-acting mechanism that can repress the expression of H-2 antigens, and that the gene(s) causing the repression are not located on chromosome 17. This mechanism is not sufficient to explain the H-2- phenotype of BM5R.9, for which an additional, cis-acting process, must be postulated. We discuss these results in the context of the regulation of expression of the major class I transplantation antigens.
摘要
我们将一个H-2-胸腺瘤（BM5R.9）与一个H-2+胸腺瘤（BW5147）进行了融合，发现许多由此产生的杂种表现出H-2-表型。在几个经过详细分析的杂种中，这种表型与稳态H-2 mRNA的缺失有关，并表现出一些不稳定性，这可能与分离物中染色体的丢失有关。我们从研究中得出结论，BM5R.9细胞表现出一种反式作用机制，该机制可以抑制H-2抗原的表达，并且导致这种抑制的基因不在17号染色体上。这种机制不足以解释BM5R.9的H-2-表型，为此必须假定存在一个额外的顺式作用过程。我们在主要I类移植抗原表达调控的背景下讨论了这些结果。
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