Then G M, Appel H, Duffield J, Taylor D M, Thies W G
J Inorg Biochem. 1986 Aug;27(4):255-70. doi: 10.1016/0162-0134(86)80066-6.
The binding of hafnium to rat serum transferrin was studied using the time differential perturbed angular correlation (TDPAC) technique. Hafnium is interesting as a toxic metal binding to transferrin because it behaves metabolically similarly to plutonium. The isotope 181Hf offers favorable access to the TDPAC-method. Samples were prepared in vivo by intravenous injection of Hf-NTA, Hf-citrate, and Hf-oxalate solutions, respectively, into Sprague-Dawley rats and in vitro by adding Hf-NTA solution to fresh rat serum. In both cases two specific electric quadrupole interactions were observed, which correspond to two well-defined binding configurations. They may be attributed to the N-terminal and the C-terminal binding site in the transferrin molecule. The 181Hf-distribution between these two binding states depends on pH, salt and hafnium concentrations, temperature, and incubation time. With a fast TDPAC-setup of four BaF2-detectors a time resolution of about 600 ps could be achieved. The specific binding configurations of 181Hf and the comparatively slow relaxation times lead to spectra of considerable accuracy.
采用时间微分扰动角关联（TDPAC）技术研究了铪与大鼠血清转铁蛋白的结合。铪作为一种与转铁蛋白结合的有毒金属很有意思，因为它在代谢方面与钚相似。同位素181铪为TDPAC方法提供了有利条件。分别通过向斯普拉格-道利大鼠静脉注射Hf-NTA、Hf-柠檬酸盐和Hf-草酸盐溶液在体内制备样品，并通过向新鲜大鼠血清中添加Hf-NTA溶液在体外制备样品。在这两种情况下，均观察到两种特定的电四极相互作用，它们对应于两种明确的结合构型。它们可能归因于转铁蛋白分子中的N端和C端结合位点。这两种结合状态之间的181铪分布取决于pH值、盐和铪的浓度、温度以及孵育时间。使用由四个BaF2探测器组成的快速TDPAC装置，可实现约600皮秒的时间分辨率。181铪的特定结合构型和相对较慢的弛豫时间导致了具有相当高准确度的光谱。
