School of Physical Sciences, Jawaharlal Nehru University, New Delhi, India.
Department of Chemistry, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India.
Biochem Biophys Res Commun. 2023 Oct 1;675:99-105. doi: 10.1016/j.bbrc.2023.06.081. Epub 2023 Jul 4.
In this study, we have fabricated a novel platform for sensing of urea using gelatin/carbon dots nanocomposite system. The sensor electrode was created by depositing the nanocomposite gel onto thin glass plates coated with indium tin oxide (ITO) using the drop casting technique. The behavior of these electrodes was investigated against a number of bioanalytes in the concentration range of 2-20 mM by cyclic voltammetry. The system was observed to be highly selective for urea with a sensitivity of 1.65 μA/mM/cm in the experimental linear range of 2-20 mM. Furthermore, the gelatin/CD-ITO electrode were also subjected to 50 KeV N ion beam irradiation with varying fluence in the range of 10 to 10 ions/cm. Sensing profile of the irradiated samples for urea suggested enhancement in sensitivity to 2 μA/mM cm, when the ion fluence was 5 × 10 ions/cm. This enhancement after irradiation suggests a clear dependence of detection on the fluence of the ion beam. The observed excellent sensitivity of radiation processed nanocomposite material can be used as an enzyme-free platform for urea detection. Additionally, the CDs showed fluorescence quenching on treatment with mere 50 μM urea suggesting the high sensitivity of the platform.
在这项研究中，我们使用明胶/碳点纳米复合材料系统制造了一种用于检测尿素的新型平台。传感器电极是通过使用滴铸技术将纳米复合材料凝胶沉积到涂有氧化铟锡（ITO）的薄玻璃板上来创建的。通过循环伏安法，研究了这些电极对浓度范围为 2-20 mM 的多种生物分析物的行为。该系统对尿素具有高度选择性，在实验线性范围内为 2-20 mM 时灵敏度为 1.65 μA/mM/cm。此外，还将明胶/CD-ITO 电极用 50 keV N 离子束以 10 至 10 离子/cm 的范围内进行不同剂量的辐照。对于尿素，辐照样品的传感曲线表明，当离子剂量为 5×10 离子/cm 时，灵敏度提高到 2 μA/mM cm。这种辐照后的增强表明检测明显依赖于离子束的剂量。辐照处理的纳米复合材料的优异灵敏度可作为无酶平台用于尿素检测。此外，CD 在处理仅 50 μM 尿素时显示出荧光猝灭，表明该平台具有高灵敏度。
