Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou, 350108, PR China.
Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou, 350108, PR China.
Biosens Bioelectron. 2023 Oct 1;237:115535. doi: 10.1016/j.bios.2023.115535. Epub 2023 Jul 16.
High entropy (HE) compounds with chemically disordered multi-cation structures have become a hot research topic because of their fascinating "cocktail effect". However, high entropy effect with the efficient photoelectric response has not been reported for photoelectrochemical (PEC) immunoassays. Herein, an innovative PEC immunoassay for the sensitive detection of prostate-specific antigen (PSA) was ingeniously constructed using hollow nanocubic (ZnCdFeMnCu)S photoactive matrices with high entropic effect via the cation exchange. Initially, a sandwich-type immunoreaction has behaved using dopamine-loaded liposome labeled with anti-PSA secondary antibodies. In the presence of PSA, addition of Triton X-100 caused the liposomal cleavage to release dopamine, which was then detected as a reduced photocurrent on (ZnCdFeMnCu)S-based photoelectrode. Under optimal condition, the PEC immunoassay showed good photocurrent responses toward target PSA with the dynamic linear range of 0.1-50 ng mL with a limit of detection of 34.1 pg mL. Significantly, this system can provide a new platform for the development of PEC immunoassays by coupling with high-entropy photoactive materials.
具有化学无序多阳离子结构的高熵（HE）化合物因其迷人的“鸡尾酒效应”而成为热门研究课题。然而，光电化学（PEC）免疫分析中尚未报道具有高效光电响应的高熵效应。在此，通过阳离子交换，使用具有高熵效应的中空纳米立方（ZnCdFeMnCu）S 光活性基体，巧妙地构建了用于灵敏检测前列腺特异性抗原（PSA）的创新型 PEC 免疫分析。最初，使用载有抗 PSA 二级抗体的多巴胺负载脂质体进行三明治型免疫反应。在 PSA 的存在下，加入 Triton X-100 会导致脂质体裂解释放多巴胺，然后在基于（ZnCdFeMnCu）S 的光电电极上检测到减少的光电流。在最佳条件下，PEC 免疫分析对目标 PSA 表现出良好的光电流响应，其动态线性范围为 0.1-50ng mL，检测限为 34.1pg mL。值得注意的是，通过与高熵光活性材料结合，该系统可为 PEC 免疫分析的发展提供新平台。
