Engineering Research Center of Glycoconjugates, Ministry of Education, Jilin Provincial Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, 130024, China.
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 6-155 Jackson Hall, Minneapolis, MN, 55455, USA.
Carbohydr Res. 2023 Oct;532:108895. doi: 10.1016/j.carres.2023.108895. Epub 2023 Jul 13.
Linear β-manno-oligosaccharides (l-β-MOS) are widely used to investigate oligo- and poly-saccharide structures and mannanolytic enzyme activities. l-β-MOS are also being used as prebiotic agents with potential bio-active properties. In this study, we developed an efficient protocol to prepare a series of l-β-MOS by hydrolyzing cassia gum (CG) using mannanolytic enzymes (endo-1,4-β-mannanase, α-galactosidases and β-glucosidases). By using medium pressure liquid chromatography (MPLC), we purified l-β-MOS with different degrees of polymerization (DPs). HPAEC-PAD, MALDI-TOF-MS and NMR studies confirmed that these l-β-MOS species ranged from 1,4-β-d-mannobiose to 1,4-β-d-mannononaose (DP 2-9) with >95% purity. Our results provide a robust approach to preparing l-β-MOS, thus enabling l-β-MOS to be further used in the fields of chemistry, life science, and nutritional food.
线性 β-甘露低聚糖（l-β-MOS）被广泛用于研究寡糖和多糖结构以及甘露聚糖酶活性。l-β-MOS 也被用作具有潜在生物活性的益生元。在这项研究中，我们开发了一种使用甘露聚糖酶（内切-1,4-β-甘露聚糖酶、α-半乳糖苷酶和β-葡萄糖苷酶）水解刺槐豆胶（CG）制备一系列 l-β-MOS 的有效方法。通过使用中压液相色谱（MPLC），我们对不同聚合度（DP）的 l-β-MOS 进行了纯化。HPAEC-PAD、MALDI-TOF-MS 和 NMR 研究证实，这些 l-β-MOS 种类从 1,4-β-d-甘露二糖到 1,4-β-d-甘露九糖（DP 2-9），纯度均>95%。我们的结果提供了一种制备 l-β-MOS 的可靠方法，从而使 l-β-MOS 能够进一步应用于化学、生命科学和营养食品领域。
