Department of Infectious Diseases, Leiden University Medical Center, Leiden, the Netherlands.
Department of Immunology, Leiden University Medical Center, Leiden, the Netherlands.
Sci Rep. 2023 Jul 18;13(1):11617. doi: 10.1038/s41598-023-38889-x.
Tuberculosis (TB) is a prevalent disease causing an estimated 1.6 million deaths and 10.6 million new cases annually. Discriminating TB disease from differential diagnoses can be complex, particularly in the field. Increased levels of complement component C1q in serum have been identified as a specific and accessible biomarker for TB disease but the source of C1q in circulation has not been identified. Here, data and samples previously collected from human cohorts, a clinical trial and a non-human primate study were used to identify cells producing C1q in circulation. Cell subset frequencies were correlated with serum C1q levels and combined with single cell RNA sequencing and flow cytometry analyses. This identified monocytes as C1q producers in circulation, with a pronounced expression of C1q in classical and intermediate monocytes and variable expression in non-classical monocytes.
结核病（TB）是一种流行疾病，每年估计导致 160 万人死亡和 1060 万例新发病例。从鉴别诊断中区分结核病可能很复杂，特别是在现场。循环中补体成分 C1q 的水平升高已被确定为结核病的特异性和可及性生物标志物，但循环中 C1q 的来源尚未确定。在这里，使用先前从人类队列、临床试验和非人类灵长类动物研究中收集的数据和样本，来鉴定循环中产生 C1q 的细胞。细胞亚群频率与血清 C1q 水平相关，并与单细胞 RNA 测序和流式细胞术分析相结合。这确定了单核细胞是循环中的 C1q 产生细胞，经典和中间单核细胞中 C1q 的表达明显，而非经典单核细胞中 C1q 的表达则不同。
