Schrijver Benjamin, Dijkstra Douwe J, Borggreven Nicole V, La Distia Nora Rina, Huijser Erika, Versnel Marjan A, van Hagen P Martin, Joosten Simone A, Trouw Leendert A, Dik Willem A
Department of Immunology Laboratory Medical Immunology Erasmus MC University Medical Center Rotterdam Rotterdam The Netherlands.
Department of Immunohematology and Blood Transfusion Leiden University Medical Center Leiden The Netherlands.
Clin Transl Immunology. 2020 Oct 16;9(10):e1196. doi: 10.1002/cti2.1196. eCollection 2020.
To examine the relation between serum C1q levels and blood type-1 interferon signature (type-1 IFN signature) in active pulmonary tuberculosis (APTB) and to determine whether combined measurement of serum C1q and type-1 IFN signature may add to the diagnosis of QuantiFERON-positive (QFT) patients with uveitis of unknown cause.
C1q was determined (ELISA) in serum from two distinct Indonesian cohorts, and in total, APTB ( = 72), QFT uveitis of unknown aetiology ( = 58), QFT uveitis ( = 51) patients and healthy controls (HC; = 73) were included. The type-1 IFN signature scores were previously determined.
Serum C1q was higher in APTB than HC ( < 0.001). APTB patients with uveitis had higher serum C1q than APTB patients without uveitis ( = 0.0207). Serum C1q correlated inversely with type-1 IFN signature scores in APTB ( = 0.0036, = 0.3526), revealing that these biomarkers for active TB disease can be mutually exclusive. Stratification of QFT patients with uveitis of unknown cause, by serum C1q and type-1 IFN signature, yielded four groups with different likelihood of suffering from active TB uveitis.
Serum C1q is elevated in APTB, especially in those cases with uveitis. We propose that combined measurement of blood type-1 IFN signature and serum C1q may provide added value in the diagnosis of active TB disease. Combined measurement of type-1 IFN signature and serum C1q in QFT patients without signs of active TB disease, but suffering from uveitis of unknown cause, may be of help to identify cases with low or high likelihood of having active TB uveitis, which may facilitate clinical management decisions.
研究活动性肺结核（APTB）患者血清C1q水平与1型干扰素特征（1型IFN特征）之间的关系，并确定血清C1q和1型IFN特征的联合检测是否有助于不明原因葡萄膜炎的QuantiFERON阳性（QFT）患者的诊断。
采用酶联免疫吸附测定（ELISA）法检测来自两个不同印度尼西亚队列的血清C1q水平，共纳入APTB患者（n = 72）、不明病因的QFT葡萄膜炎患者（n = 58）、QFT葡萄膜炎患者（n = 51）和健康对照（HC；n = 73）。1型IFN特征评分先前已测定。
APTB患者的血清C1q水平高于HC（P < 0.001）。合并葡萄膜炎的APTB患者血清C1q水平高于未合并葡萄膜炎的APTB患者（P = 0.0207）。APTB患者血清C1q水平与1型IFN特征评分呈负相关（P = 0.0036，r = 0.3526），表明这些活动性结核病生物标志物可能相互排斥。根据血清C1q和1型IFN特征对不明原因葡萄膜炎的QFT患者进行分层，得到四组患活动性结核性葡萄膜炎可能性不同的患者。
APTB患者血清C1q水平升高，尤其是合并葡萄膜炎的患者。我们认为，联合检测血液1型IFN特征和血清C1q可能为活动性结核病的诊断提供附加价值。对无活动性结核病迹象但患有不明原因葡萄膜炎的QFT患者联合检测1型IFN特征和血清C1q，可能有助于识别患活动性结核性葡萄膜炎可能性低或高的病例，从而有助于临床管理决策。
