Department of Physiology, Medicine and Nursing Faculty, University of the Basque Country UPV/EHU, Leioa, Spain.
Department of Pharmacology, Medicine and Nursing Faculty, University of the Basque Country UPV/EHU, Leioa, Spain.
Methods Mol Biol. 2023;2687:45-55. doi: 10.1007/978-1-0716-3307-6_4.
The development of schizophrenia-like rodent models is still a major challenge for the study of this mental disorder. Schizophrenia and other neuropsychiatric disorders are thought to be triggered by multiple factors, and furthermore, the genetic component of schizophrenia is highly complex. The edition of one single gene for mimicking some of the symptoms of the disorder could cause unintended mutations that could influence animal's behavior making it difficult to study. Since 2013, CRISPR-Cas gene-editing technology has been a great improvement in the specificity of transgenic model generation because of its speed, efficiency, cost, and apparent ease. This protocol describes a simple method to generate a knockout mouse model using CRISPR technology, which can be applied to any gene presumably involved in the development of schizophrenia and other neuropsychiatric disorders.
精神分裂症样啮齿动物模型的发展仍然是研究这种精神障碍的主要挑战。精神分裂症和其他神经精神疾病被认为是由多种因素引发的，此外，精神分裂症的遗传成分非常复杂。编辑一个单一的基因来模拟该疾病的一些症状可能会导致意想不到的突变，从而影响动物的行为，使其难以研究。自 2013 年以来，CRISPR-Cas 基因编辑技术在转基因模型生成的特异性方面有了很大的提高，因为它速度快、效率高、成本低、操作简单。本方案描述了一种使用 CRISPR 技术生成基因敲除小鼠模型的简单方法，该方法可应用于任何可能参与精神分裂症和其他神经精神疾病发展的基因。
