Raymond P A
J Neurosci. 1986 Sep;6(9):2479-88. doi: 10.1523/JNEUROSCI.06-09-02479.1986.
Quantitative, computer-assisted autoradiography was used to assess the relative rate and pattern of growth of retina and tectum in larval and early juvenile goldfish. 3H-thymidine was used to mark the boundary of retina and tectum, and the location of this boundary was charted as the eye and brain grew and added more cells. The pattern of growth is at all times discordant. The original (larval) retina becomes surrounded by annuli of new tissue, whereas the larval tectum remains adjacent to the rostral edge as crescents of new tissue are added to the caudal end. After 2 years of growth, more than 95% of the total surface area of retina and tectum in goldfish derives from cells born after larval stages. Computer-aided reconstructions of 3H-thymidine labeled retina and tecta were used to predict the direction and magnitude of displacement of the retinotopic map. It was estimated that retinal terminals can shift 1.5-1.8 mm caudally at a rate of 5 micron/d during the first 2 years of growth. The terminals that move the farthest are those from temporal retina that project to rostral tectum. The magnitude and direction of the predicted movements matches certain features of HRP-filled retinal axons that others have assumed represented the history of displacements of the terminal arbors.
采用定量、计算机辅助放射自显影技术评估幼体和幼年期早期金鱼视网膜和视顶盖的相对生长速率和模式。用³H-胸腺嘧啶核苷标记视网膜和视顶盖的边界，并随着眼睛和大脑的生长以及细胞的增加绘制该边界的位置。生长模式始终不一致。原始（幼体）视网膜被新组织的环带包围，而幼体视顶盖随着新组织的月牙形在尾端增加而仍与吻端边缘相邻。生长2年后，金鱼视网膜和视顶盖的总表面积超过95%来自幼体阶段后产生的细胞。使用³H-胸腺嘧啶核苷标记的视网膜和视顶盖的计算机辅助重建来预测视网膜拓扑图位移的方向和大小。据估计，在生长的前2年中，视网膜终末可以以5微米/天的速度向尾侧移动1.5 - 1.8毫米。移动最远的终末是来自颞侧视网膜并投射到吻侧视顶盖的终末。预测运动的大小和方向与其他人认为代表终末分支位移历史的HRP填充视网膜轴突的某些特征相匹配。
