维甲酸对斑马鱼腹侧视网膜的发育是必需的。
Retinoic acid is necessary for development of the ventral retina in zebrafish.
作者信息
Marsh-Armstrong N, McCaffery P, Gilbert W, Dowling J E, Dräger U C
机构信息
Program in Neuroscience, Harvard University, Boston, MA 02115.
出版信息
Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7286-90. doi: 10.1073/pnas.91.15.7286.
In the embryonic zebrafish retina, as in other vertebrates, retinoic acid is synthesized from retinaldehyde by two different dehydrogenases, one localized dorsally, the other primarily ventrally. Early in eye development only the ventral enzyme is present. Citral competitively inhibits the ventral enzyme in vitro and decreases the production of retinoic acid in the ventral retina in vivo. Treatment of neurula-stage zebrafish embryos with citral during the formation of the eye primordia results in eyes lacking a ventral retina. This defect can be partially rescued by retinoic acid. The results demonstrate that synthesis of retinoic acid can be selectively inhibited in vivo and suggest that retinoic acid is necessary for the proper development of the ventral retina.
与其他脊椎动物一样，在斑马鱼胚胎视网膜中，视黄酸由视黄醛通过两种不同的脱氢酶合成，一种定位于背侧，另一种主要定位于腹侧。在眼睛发育早期，仅存在腹侧酶。柠檬醛在体外竞争性抑制腹侧酶，并在体内降低腹侧视网膜中视黄酸的产生。在眼原基形成期间用柠檬醛处理神经胚期斑马鱼胚胎会导致眼睛缺乏腹侧视网膜。这种缺陷可以通过视黄酸部分挽救。结果表明，视黄酸的合成在体内可以被选择性抑制，并提示视黄酸对于腹侧视网膜的正常发育是必需的。
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