Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, Tulane Cancer Center, New Orleans, LA, USA.
Current address: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.
Methods Mol Biol. 2023;2677:151-171. doi: 10.1007/978-1-0716-3259-8_9.
Developments in single-cell technology have considerably changed the way we study biology. Significant efforts have been made over the last few years to build comprehensive cell-type-specific transcriptomic atlases for a wide range of tissues in several model organisms in order to discover cell-type-specific markers and drivers of gene expression. One such tissue is the ovary of the fruit-fly Drosophila melanogaster, which is a popular model system with wide-ranging applications in the study of both development and disease. Three independent studies have recently produced comprehensive maps of cell-type-specific gene expression that describe both spatiotemporal regulation of the process of oogenesis and unique transcriptomic profiles of different cell types that constitute the ovary. In this chapter, we outlined the wet-lab protocol that was followed in our recent study for sample preparation and reanalyze the resultant dataset to discuss the benchmarks in data analysis, which are fundamental to comprehensive curation of the single-cell dataset representing the fly ovary.
单细胞技术的发展极大地改变了我们研究生物学的方式。在过去的几年中，人们做出了巨大的努力来构建全面的细胞类型特异性转录组图谱，涵盖了多种模式生物的广泛组织，以发现细胞类型特异性标记物和基因表达的驱动因素。果蝇的卵巢就是这样一种组织，它是一种流行的模型系统，在发育和疾病研究中有着广泛的应用。最近有三项独立的研究产生了全面的细胞类型特异性基因表达图谱，描述了卵子发生过程的时空调节以及构成卵巢的不同细胞类型的独特转录组特征。在本章中，我们概述了我们最近的研究中用于样本制备的湿实验方案，并重新分析了结果数据集，以讨论数据分析的基准，这对于全面整理代表果蝇卵巢的单细胞数据集是至关重要的。
