Department of Obstetrics, Gynecology and Reproductive Science, Center for Reproductive Sciences, Eli and Edythe Broad Center for Regeneration Medicine and Stem Cell Research, UCSF, San Francisco, CA, USA.
Genomics and Cell Characterization Core Facility, Institute of Molecular Biology, Eugene, OR, USA.
Methods Mol Biol. 2023;2677:203-219. doi: 10.1007/978-1-0716-3259-8_12.
Recent advances in tissue clearing methodologies have enabled three-dimensional (3D) visualization of the ovary and, consequently, in-depth exploration of the dynamic changes occurring at the single-cell level. Here we describe methods for whole-mount immunofluorescence, clearing, imaging, and analysis of whole ovarian tissue in 3D throughout murine development and aging.
近年来，组织透明化方法的进展使得人们能够对卵巢进行三维（3D）可视化，从而深入探究单细胞水平上发生的动态变化。在这里，我们描述了在整个小鼠发育和衰老过程中对整个卵巢组织进行 3D 全组织免疫荧光、透明化、成像和分析的方法。
