School of Life Sciences, Joseph Banks Laboratories, University of Lincoln, Lincoln LN6 7DL, UK.
School of Chemistry, Joseph Banks Laboratories, University of Lincoln, Lincoln LN6 7DL, UK.
J R Soc Interface. 2023 Jul;20(204):20230154. doi: 10.1098/rsif.2023.0154. Epub 2023 Jul 19.
The purpose of this study is to examine and to compare the ionic composition of the haemolymph and the ear fluid of seven species of katydids (Orthoptera: Tettigoniidae) with the aim of providing from a biochemical perspective a preliminary assessment for an insect liquid contained in the auditory organ of katydids with a hearing mechanism reminiscent of that found in vertebrates. A multi-element trace analysis by inductively coupled plasma optical-emission spectrometry was run for 16 elements for the ear liquid of seven species and the haemolymph of six of them. Based on the obtained results, it can be recognized that the ionic composition is variable among the studied insects, but sodium (Na), potassium (K), calcium (Ca) and magnesium (Mg) are the most prominent of the dissolved inorganic cations. However, the ion concentrations between the two fluids are considerably different and the absence or low concentration of Ca is a noticeable feature in the inner ear liquid. A potential relationship between the male courtship song peak frequency and the total ion (Na, K, Mg and Ca) concentration of the inner ear liquid is also reported.
本研究旨在分析和比较七种螽斯（直翅目：螽斯科）的血淋巴和耳液的离子组成，以期从生物化学的角度对螽斯听觉器官中的昆虫液体进行初步评估，因为该听觉器官的机制类似于脊椎动物。通过电感耦合等离子体光学发射光谱法对七种耳液和其中六种血淋巴中的 16 种元素进行了多元素痕量分析。基于获得的结果，可以识别出在所研究的昆虫中，离子组成是可变的，但溶解无机阳离子中最突出的是钠（Na）、钾（K）、钙（Ca）和镁（Mg）。然而，两种液体之间的离子浓度差异很大，内耳液中 Ca 的缺失或低浓度是一个显著特征。还报告了雄性求偶鸣声峰值频率与内耳液中总离子（Na、K、Mg 和 Ca）浓度之间的潜在关系。
