School of Biological Science and Medical Engineering, Beihang University, Beijing, China.
Institute of Environmental Biology and Life Support Technology, Beihang University, Beijing, China.
Biotechnol J. 2023 Nov;18(11):e2300135. doi: 10.1002/biot.202300135. Epub 2023 Jul 28.
Extraction of DNA is a key step in molecular biology experiments and important for counting tiny microbial individuals. Direct boiling and mechanical cell lysis like glass beads are two independent physical extraction methods, thus crossing the barriers of thresholds of magnitude in popular reagent kits or traditional spread plate method when non-equilibrium phenomenon is ongoing. The two approaches above were combined to generate a new one. In three typical microbial species, direct boiling with glass beads significantly increased the purity of DNA solution compared with some other methods (p < 0.05). The qPCR results of them were closer to direct microscopy counting than some other methods. Therefore, it provides a new choice in extracting bacterial DNA for specific circumstances.
DNA 提取是分子生物学实验的关键步骤，对于计数微小的微生物个体尤为重要。直接煮沸和玻璃珠等机械细胞裂解是两种独立的物理提取方法，因此在非平衡现象持续存在时，跨越了流行试剂盒或传统平板法的阈值幅度障碍。上述两种方法结合起来产生了一种新方法。在三种典型的微生物物种中，与其他方法相比，直接煮沸和玻璃珠显著提高了 DNA 溶液的纯度（p < 0.05）。它们的 qPCR 结果比其他方法更接近直接显微镜计数。因此，它为特定情况下提取细菌 DNA 提供了新的选择。
