Lin Ziyan, Wang Kun, Feng Jing
Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning, 530008, China.
College of Life Science, Northeast Forestry University, Harbin, 150040, China.
BMC Plant Biol. 2025 Apr 29;25(1):559. doi: 10.1186/s12870-025-06582-y.
A strain of Rhodococcus ruber was isolated from the rhizosphere of Spartina alterniflora. The VOCs released by this strain effectively promote the growth of Arabidopsis thaliana and inhibit several plant pathogenic fungi, including Bipolaris sorokiniana, Cryphonectria parasitica, Fusarium oxysporum, Fusarium pseudograminearum, and Plectosphaerella cucumerina. SPME/GC-MS analysis revealed that the strain produces dimethyl disulfide (DMDS) and dimethyl trisulfide (DMTS), with DMDS being the predominant component of the volatile organic compounds (VOCs). It was observed that the growth of A. thaliana was enhanced under fumigation with DMDS and DMTS. Furthermore, these compounds effectively inhibited the aforementioned plant pathogenic fungi, with DMTS demonstrating a lethal effect on plant pathogenic fungi. Previous studies have confirmed that DMDS and DMTS promote the growth of A. thaliana. In this study, we found that DMTS could significantly enhance plant growth and inhibit plant pathogenic fungi even at low dosages. Transcriptome analysis indicated that the growth-related genes of A. thaliana were significantly upregulated in response to treatment with VOCs from R. ruber. Additionally, VOCs induced changes in multiple plant defense response genes and promoted the C4 pathway.
从互花米草的根际分离出一株红球菌。该菌株释放的挥发性有机化合物（VOCs）能有效促进拟南芥的生长，并抑制几种植物病原真菌，包括禾旋孢腔菌、栗疫病菌、尖孢镰刀菌、小麦假禾谷镰刀菌和黄瓜炭疽病菌。固相微萃取/气相色谱-质谱联用（SPME/GC-MS）分析表明，该菌株产生二甲基二硫醚（DMDS）和二甲基三硫醚（DMTS），其中DMDS是挥发性有机化合物（VOCs）的主要成分。观察到用DMDS和DMTS熏蒸后拟南芥的生长得到增强。此外，这些化合物有效抑制了上述植物病原真菌，其中DMTS对植物病原真菌表现出致死作用。先前的研究已证实DMDS和DMTS能促进拟南芥的生长。在本研究中，我们发现即使在低剂量下，DMTS也能显著促进植物生长并抑制植物病原真菌。转录组分析表明，拟南芥中与生长相关的基因在受到来自红球菌的VOCs处理后显著上调。此外，VOCs诱导了多个植物防御反应基因的变化并促进了C4途径。
