Li Bingxuan, Zhou Gen, Li Yanbin, Chen Xueting, Yang Huiting, Li Yan, Zhu Minhua, Li Libei
Department of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Jurong, China.
Key laboratory of Quality Improvement of Agriculture Products of Zhejiang Province, College of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, China.
Front Plant Sci. 2023 Jul 3;14:1147932. doi: 10.3389/fpls.2023.1147932. eCollection 2023.
Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (R-SNAREs) mainly promoted the assembly of the SNARE complex to drive the final membrane fusion step of membrane transport. Previous research on R-SNAREs has mainly focused on development and growth and has rarely been involved in abiotic stress, especially in cotton. Here, we performed a comprehensive analysis of genes in upland cotton. In total, 51 genes across six phylogenetic groups were unevenly distributed on 21 chromosomes. Cis elements related to plant growth and response to abiotic stress responses were found in the promoter region of . Nine genes were obviously upregulated under drought stress conditions by RNA-seq and qRT-PCR analysis. Among them, might be the key candidate gene contributing to drought stress tolerance in cotton by virus-induced gene silencing (VIGS) assay. These results provide valuable insights for the functional analysis of cotton in response to drought stress and highlight potential beneficial genes for genetic improvement and breeding in cotton.
可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体（R-SNAREs）主要促进SNARE复合体的组装，以驱动膜运输的最终膜融合步骤。先前对R-SNAREs的研究主要集中在发育和生长方面，很少涉及非生物胁迫，尤其是在棉花中。在此，我们对陆地棉中的基因进行了全面分析。总共，六个系统发育组中的51个基因不均匀地分布在21条染色体上。在的启动子区域发现了与植物生长和对非生物胁迫反应相关的顺式元件。通过RNA-seq和qRT-PCR分析，九个基因在干旱胁迫条件下明显上调。其中，通过病毒诱导基因沉默（VIGS）试验，可能是有助于棉花耐旱性的关键候选基因。这些结果为棉花响应干旱胁迫的功能分析提供了有价值的见解，并突出了棉花遗传改良和育种的潜在有益基因。
