State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
The National keys laboratory of Basic Sciences of Stomatology of Kinshasa University, School of Medical University of Kinshasa, Kinshasa, Democratic Republic of Congo.
Head Neck. 2023 Sep;45(9):2161-2172. doi: 10.1002/hed.27464. Epub 2023 Jul 19.
Cysteine-rich protein 2 (CSRP2) is discovered as oncogene. The study aims to investigate the clinical significance and potential mechanism of CSRP2 in head and neck squamous cell carcinoma (HNSCC).
CSRP2 expression was explored by immunohistochemistry tissue microarrays and Western blotting in HNSCC. The effect of CSRP2 on the cancer stemness and epithelial-to-mesenchymal transition (EMT) of HNSCC cells was investigated by sphere formation, wound healing, and transwell assays. The vitro and vivo experiments revealed that CSRP2 modulated cancer stemness and EMT phenotypes in HNSCC.
CSRP2 was overexpressed in HNSCC patients and presented poor prognosis. CSRP2 knockdown inhibited the migration and invasion ability of the HNSCC cells. And CSRP2 expression was closely associated with CSCs markers, EMT-transcription factor, new oncoprotein, and immune checkpoint.
The overexpression of CSRP2 indicates poor prognosis and plays a key role in maintaining the cancer cell stemness and EMT.
富含半胱氨酸蛋白 2（CSRP2）被发现是一种癌基因。本研究旨在探讨 CSRP2 在头颈部鳞状细胞癌（HNSCC）中的临床意义和潜在机制。
采用免疫组织化学组织微阵列和 Western blot 检测 HNSCC 中 CSRP2 的表达。通过球体形成、划痕愈合和 Transwell 检测，研究 CSRP2 对头颈部鳞状细胞癌细胞癌干细胞特性和上皮间质转化（EMT）的影响。体外和体内实验揭示了 CSRP2 对头颈部鳞状细胞癌癌干细胞特性和 EMT 表型的调节作用。
CSRP2 在 HNSCC 患者中过表达，预示着预后不良。CSRP2 敲低抑制了 HNSCC 细胞的迁移和侵袭能力。CSRP2 的表达与 CSCs 标志物、EMT 转录因子、新癌蛋白和免疫检查点密切相关。
CSRP2 的过表达表明预后不良，并在维持癌细胞的干性和 EMT 中发挥关键作用。
