Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
Bioresour Technol. 2023 Oct;386:129511. doi: 10.1016/j.biortech.2023.129511. Epub 2023 Jul 17.
The nitrogen removal performance, nitrogen metabolism enzyme activities, and antioxidant response of four Chlorella species (Chlorella sp., Chlorella vulgaris, Chlorella sorokiniana, and Chlorella protothecoides) were compared under different light: dark (L:D) photoperiods during treating mariculture wastewater. The increase of light duration in the range of 8L:16D to 16L:8D was beneficial to the chlorophyll synthesis of selected four Chlorella species. Chlorella vulgaris was the most effective to treat mariculture wastewater than Chlorella sp., Chlorella sorokiniana, and Chlorella protothecoides. and its microalgae density, photosynthetic activity, and nitrogen metabolism enzyme activity were higher than those of the other three Chlorella species. An obvious oxidative stress in microalgal cells was under 20L:4D photoperiod, which led to a decrease in photosynthetic activity and nitrogen metabolizing enzyme activity. Among the four Chlorella species, Chlorella protothecoides had the highest degree of light-induced stress and ROS accumulation. This study can provide suitable microalgae and optimal photoperiod for treating mariculture wastewater.
四种小球藻（普通小球藻、蛋白核小球藻、盐藻和绿色巴夫藻）在不同光照：黑暗（L:D）光周期下处理海水养殖废水的脱氮性能、氮代谢酶活性和抗氧化响应进行了比较。光照时间从 8L:16D 增加到 16L:8D 有利于所选四种小球藻的叶绿素合成。与普通小球藻、盐藻和绿色巴夫藻相比，蛋白核小球藻对海水养殖废水的处理效果最好，其微藻密度、光合作用和氮代谢酶活性均高于其他三种小球藻。在 20L:4D 光周期下，微藻细胞受到明显的氧化应激，导致光合作用和氮代谢酶活性下降。在这四种小球藻中，绿色巴夫藻受到的光诱导应激和 ROS 积累程度最高。本研究可为海水养殖废水处理提供适宜的微藻和最佳光周期。
