嗜热栖热菌HB8 5S rRNA晶体的电子显微镜研究。

Electron microscopic study of 5S rRNA crystals from Thermus thermophilus HB8.

作者信息

Morikawa K, Fujiyoshi Y, Ishizuka K, Sugiyama J, Kawakami M, Takemura S

出版信息

J Microsc. 1986 May;142(Pt 2):247-58. doi: 10.1111/j.1365-2818.1986.tb02761.x.

DOI:10.1111/j.1365-2818.1986.tb02761.x

Abstract

Several types of crystals were grown from 5S rRNA, which was purified from the highly thermophilic bacterium, Thermus thermophilus HB8. Crystal lattice parameters were determined by X-ray and electron diffraction. One type of crystal was suitable for electron microscopy after staining with uranyl acetate. Two projections derived from micrographs taken at different tilt angles were processed for image analysis. This result enabled us to deduce the arrangement of molecules within the crystal lattice.

摘要

从嗜热栖热菌HB8中纯化得到的5S rRNA培养出了几种晶体。通过X射线和电子衍射确定了晶格参数。其中一种晶体在用醋酸双氧铀染色后适合进行电子显微镜观察。对从不同倾斜角度拍摄的显微照片得到的两个投影进行了图像处理分析。这一结果使我们能够推断出晶格内分子的排列方式。