Lorenz S, Perbandt M, Lippmann C, Moore K, DeLucas L J, Betzel C, Erdmann V A
Institut für Biochemie, Freie Universität Berlin, Thielallee 63, 14195 Berlin, Germany.
Acta Crystallogr D Biol Crystallogr. 2000 Apr;56(Pt 4):498-500. doi: 10.1107/s0907444900001736.
Thermus flavus 5S rRNA with a molecular weight of about 40 kDa was modified at the 5' and 3' ends. Crystals were obtained under earth and microgravity conditions. The best crystals were obtained during NASA space mission STS 94. For the first time, it was possible to collect a complete data set from 5S rRNA crystals to 7.8 A resolution and to assign the space group as R32, with unit-cell parameters a = b = 110.3, c = 387.6 A, alpha = beta = 90, gamma = 120 degrees.
分子量约为40 kDa的嗜热栖热菌5S rRNA在5'和3'末端进行了修饰。在地面和微重力条件下获得了晶体。最佳晶体是在美国国家航空航天局（NASA）STS 94太空任务期间获得的。首次有可能从5S rRNA晶体收集到分辨率为7.8 Å的完整数据集，并确定其空间群为R32，晶胞参数为a = b = 110.3，c = 387.6 Å，α = β = 90°，γ = 120°。
