Alcarranza Manuel, Villegas Isabel, Recio Rocío, Muñoz-García Rocío, Fernández Inmaculada, Alarcón-de-la-Lastra Catalina
Department of Pharmacology, Faculty of Pharmacy, Universidad de Sevilla, 41012 Sevilla, Spain.
Instituto de Biomedicina de Sevilla, IBiS (Universidad de Sevilla, HUVR, Junta de Andalucía, CSIC), Seville, Spain.
Food Funct. 2023 Jul 31;14(15):7270-7283. doi: 10.1039/d3fo02009f.
The aim of this study was to develop an optimal synthetic route to obtain natural ()-8-methylsulfinyloctyl isothiocyanate ((R)-8-OITC), present in watercress, based on the "DAG methodology" as well as to evaluate its potential antioxidant and immunomodulatory effects, exploring possible signaling pathways that could be involved in an model of murine peritoneal macrophages stimulated with LPS. Treatment with (R)-8-OITC inhibited the levels of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6, IL-17 and IL-18), intracellular ROS production and expression of pro-inflammatory enzymes (COX-2, iNOS and mPGES-1) through modulation of the expression of Nrf2, MAPKs (p38, JNK and ERK) and JAK/STAT, and the canonical and non-canonical pathways of the inflammasome. Taking all these together, our results provide a rapid and cost-effective synthetic route to obtain natural (R)-8-OITC and demonstrate that it could be a potential nutraceutical candidate for managing immuno-inflammatory pathologies. Therefore, further trials are warranted.
本研究的目的是基于“DAG 方法”开发一种优化的合成路线，以获得存在于西洋菜中的天然（）-8-甲基亚磺酰基辛基异硫氰酸酯（（R）-8-OITC），并评估其潜在的抗氧化和免疫调节作用，探索在 LPS 刺激的小鼠腹腔巨噬细胞模型中可能涉及的信号通路。用（R）-8-OITC 处理可通过调节 Nrf2、MAPKs（p38、JNK 和 ERK）以及 JAK/STAT 的表达，以及炎性小体的经典和非经典途径，抑制促炎细胞因子（IL-1β、TNF-α、IL-6、IL-17 和 IL-18）的水平、细胞内 ROS 的产生以及促炎酶（COX-2、iNOS 和 mPGES-1）的表达。综合所有这些结果，我们的研究结果提供了一种快速且经济高效的合成路线来获得天然（R）-8-OITC，并证明它可能是一种用于管理免疫炎症性疾病的潜在营养保健品候选物。因此，有必要进行进一步的试验。
