• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

（）-萝卜硫素对脂多糖激活的小鼠免疫细胞的免疫调节作用：分子信号通路及组蛋白标记的表观遗传变化

Immunomodulatory Effects of ()-Sulforaphane on LPS-Activated Murine Immune Cells: Molecular Signaling Pathways and Epigenetic Changes in Histone Markers.

作者信息

Alcarranza Manuel, Villegas Isabel, Muñoz-García Rocío, Recio Rocío, Fernández Inmaculada, Alarcón-de-la-Lastra Catalina

机构信息

Department of Pharmacology, Faculty of Pharmacy, Universidad de Sevilla, 41012 Sevilla, Spain.

Departamento de Química Orgánica y Farmacéutica, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain.

出版信息

Pharmaceuticals (Basel). 2022 Aug 4;15(8):966. doi: 10.3390/ph15080966.

DOI:10.3390/ph15080966
PMID:36015113
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9414446/
Abstract

The aim of this study was to explore the immunomodulatory effects of the natural enantiomer ()-Sulforaphane (SFN) and the possible signaling pathways involved in an ex vivo model of LPS-stimulated murine peritoneal macrophages. Furthermore, we studied the epigenetic changes induced by ()-SFN as well as the post-translational modifications of histone H3 (H3K9me3 and H3K18ac) in relation to the production of cytokines in murine splenocytes after LPS stimulation. ()-SFN was able to modulate the inflammatory response and oxidative stress induced by LPS stimulation in murine peritoneal macrophages through the inhibition of reactive oxygen species (ROS), nitric oxide (NO) and cytokine (IL-1β, IL-6, IL-17, IL-18 and TNF-α) production by down-regulating the expression of pro-inflammatory enzymes (iNOS, COX-2 and mPGES-1). We also found that activation of the Nrf-2/HO-1 axis and inhibition of the JAK2/STAT-3, MAPK, canonical and non-canonical inflammasome signaling pathways could have been responsible for the immunomodulatory effects of ()-SFN. Furthermore, ()-SFN modulated epigenetic modifications through histone methylation (H3K9me3) and deacetylation (H3K18ac) in LPS-activated spleen cells. Collectively, our results suggest that ()-SFN could be a promising epinutraceutical compound for the management of immunoinflammatory diseases.

摘要

本研究的目的是在脂多糖（LPS）刺激的小鼠腹腔巨噬细胞体外模型中，探究天然对映体（）-萝卜硫素（SFN）的免疫调节作用以及可能涉及的信号通路。此外，我们研究了（）-SFN诱导的表观遗传变化以及LPS刺激后小鼠脾细胞中细胞因子产生相关的组蛋白H3的翻译后修饰（H3K9me3和H3K18ac）。（）-SFN能够通过下调促炎酶（诱导型一氧化氮合酶、环氧化酶-2和微粒体前列腺素E合酶-1）的表达，抑制活性氧（ROS）、一氧化氮（NO）和细胞因子（白细胞介素-1β、白细胞介素-6、白细胞介素-17、白细胞介素-18和肿瘤坏死因子-α）的产生，从而调节LPS刺激的小鼠腹腔巨噬细胞中的炎症反应和氧化应激。我们还发现，Nrf-2/HO-1轴的激活以及JAK2/STAT-3、丝裂原活化蛋白激酶、经典和非经典炎性小体信号通路的抑制可能是（）-SFN免疫调节作用的原因。此外，（）-SFN通过LPS激活的脾细胞中的组蛋白甲基化（H3K9me3）和去乙酰化（H3K18ac）调节表观遗传修饰。总的来说，我们的结果表明，（）-SFN可能是一种用于管理免疫炎症性疾病的有前景的表观营养化合物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/470f2d44c942/pharmaceuticals-15-00966-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/06f7b995a16e/pharmaceuticals-15-00966-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/31bf7029b3bb/pharmaceuticals-15-00966-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/a2a03d62d0f8/pharmaceuticals-15-00966-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/b63c06fef5ec/pharmaceuticals-15-00966-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/b229c52b6078/pharmaceuticals-15-00966-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/e235c40c9c47/pharmaceuticals-15-00966-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/f78d90850e39/pharmaceuticals-15-00966-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/7a53d7ce8817/pharmaceuticals-15-00966-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/9fe142254d78/pharmaceuticals-15-00966-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/470f2d44c942/pharmaceuticals-15-00966-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/06f7b995a16e/pharmaceuticals-15-00966-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/31bf7029b3bb/pharmaceuticals-15-00966-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/a2a03d62d0f8/pharmaceuticals-15-00966-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/b63c06fef5ec/pharmaceuticals-15-00966-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/b229c52b6078/pharmaceuticals-15-00966-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/e235c40c9c47/pharmaceuticals-15-00966-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/f78d90850e39/pharmaceuticals-15-00966-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/7a53d7ce8817/pharmaceuticals-15-00966-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/9fe142254d78/pharmaceuticals-15-00966-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84f6/9414446/470f2d44c942/pharmaceuticals-15-00966-g010.jpg

相似文献

1
Immunomodulatory Effects of ()-Sulforaphane on LPS-Activated Murine Immune Cells: Molecular Signaling Pathways and Epigenetic Changes in Histone Markers.（）-萝卜硫素对脂多糖激活的小鼠免疫细胞的免疫调节作用：分子信号通路及组蛋白标记的表观遗传变化
Pharmaceuticals (Basel). 2022 Aug 4;15(8):966. doi: 10.3390/ph15080966.
2
Effects of Oleacein, a New Epinutraceutical Bioproduct from Extra Virgin Olive Oil, in LPS-Activated Murine Immune Cells.油橄榄苦素（一种来自特级初榨橄榄油的新型营养保健品生物制品）对脂多糖激活的小鼠免疫细胞的影响。
Pharmaceuticals (Basel). 2022 Oct 28;15(11):1338. doi: 10.3390/ph15111338.
3
()-8-Methylsulfinyloctyl isothiocyanate from inhibits LPS-induced immunoinflammatory responses in mouse peritoneal macrophages: chemical synthesis and molecular signaling pathways involved.来自[具体来源未明确]的（）-8-甲基亚磺酰辛基异硫氰酸酯抑制小鼠腹腔巨噬细胞中脂多糖诱导的免疫炎症反应：化学合成及相关分子信号通路
Food Funct. 2023 Jul 31;14(15):7270-7283. doi: 10.1039/d3fo02009f.
4
Anti-Inflammatory Effect of Sulforaphane on LPS-Activated Microglia Potentially through JNK/AP-1/NF-κB Inhibition and Nrf2/HO-1 Activation.莱菔硫烷通过抑制 JNK/AP-1/NF-κB 和激活 Nrf2/HO-1 对 LPS 激活的小胶质细胞的抗炎作用。
Cells. 2019 Feb 22;8(2):194. doi: 10.3390/cells8020194.
5
(-)-Methyl-Oleocanthal, a New Oleocanthal Metabolite Reduces LPS-Induced Inflammatory and Oxidative Response: Molecular Signaling Pathways and Histones Epigenetic Modulation.(-)-甲基油橄榄苦素，一种新的油橄榄苦素代谢产物，可减轻脂多糖诱导的炎症和氧化反应：分子信号通路与组蛋白表观遗传调控
Antioxidants (Basel). 2021 Dec 27;11(1):56. doi: 10.3390/antiox11010056.
6
Epigenetic Modulation of TLR4 Expression by Sulforaphane Increases Anti-Inflammatory Capacity in Porcine Monocyte-Derived Dendritic Cells.萝卜硫素对TLR4表达的表观遗传调控增强了猪单核细胞衍生树突状细胞的抗炎能力。
Biology (Basel). 2021 May 31;10(6):490. doi: 10.3390/biology10060490.
7
Sulforaphane alleviates lung ischemia‑reperfusion injury through activating Nrf‑2/HO‑1 signaling.萝卜硫素通过激活Nrf-2/HO-1信号通路减轻肺缺血-再灌注损伤。
Exp Ther Med. 2023 Apr 20;25(6):265. doi: 10.3892/etm.2023.11964. eCollection 2023 Jun.
8
Sulforaphane Protects Cells against Lipopolysaccharide-Stimulated Inflammation in Murine Macrophages.萝卜硫素保护细胞免受脂多糖刺激的小鼠巨噬细胞炎症反应。
Antioxidants (Basel). 2019 Nov 21;8(12):577. doi: 10.3390/antiox8120577.
9
LPS-induced expression of CD14 in the TRIF pathway is epigenetically regulated by sulforaphane in porcine pulmonary alveolar macrophages.在猪肺泡巨噬细胞中，萝卜硫素通过表观遗传调控TRIF途径中脂多糖诱导的CD14表达。
Innate Immun. 2016 Nov;22(8):682-695. doi: 10.1177/1753425916669418. Epub 2016 Sep 30.
10
Sulforaphane attenuates microglia-mediated neuronal necroptosis through down-regulation of MAPK/NF-κB signaling pathways in LPS-activated BV-2 microglia.萝卜硫素通过下调 LPS 激活的 BV-2 小胶质细胞中 MAPK/NF-κB 信号通路来减轻小胶质细胞介导的神经元坏死性凋亡。
Pharmacol Res. 2018 Jul;133:218-235. doi: 10.1016/j.phrs.2018.01.014. Epub 2018 Jan 31.

引用本文的文献

1
The Multifaceted Health Benefits of Broccoli-A Review of Glucosinolates, Phenolics and Antimicrobial Peptides.西兰花的多方面健康益处——硫代葡萄糖苷、酚类和抗菌肽综述
Molecules. 2025 May 22;30(11):2262. doi: 10.3390/molecules30112262.
2
Immunomodulatory Effects and Regulatory Mechanisms of ()-6-HITC, an Isothiocyanate from Wasabi (), in an Mouse Model of LPS-Induced Inflammation.山葵中异硫氰酸酯（）-6-HITC在脂多糖诱导的炎症小鼠模型中的免疫调节作用及调控机制
J Agric Food Chem. 2024 Oct 2;72(39):21520-21532. doi: 10.1021/acs.jafc.4c02943. Epub 2024 Sep 19.
3
Modulation of immune cum inflammatory pathway by earthworm granulation tissue extract in wound healing of diabetic rabbit model.

本文引用的文献

1
An overview of the development of EED inhibitors to disable the PRC2 function.使PRC2功能失活的EED抑制剂的研发概述。
RSC Med Chem. 2021 Oct 21;13(1):39-53. doi: 10.1039/d1md00274k. eCollection 2022 Jan 27.
2
(-)-Methyl-Oleocanthal, a New Oleocanthal Metabolite Reduces LPS-Induced Inflammatory and Oxidative Response: Molecular Signaling Pathways and Histones Epigenetic Modulation.(-)-甲基油橄榄苦素，一种新的油橄榄苦素代谢产物，可减轻脂多糖诱导的炎症和氧化反应：分子信号通路与组蛋白表观遗传调控
Antioxidants (Basel). 2021 Dec 27;11(1):56. doi: 10.3390/antiox11010056.
3
Diterpenoids from the Brown Alga and Their Anti-Inflammatory Activity.
蚯蚓肉芽组织提取物对糖尿病兔模型伤口愈合中免疫及炎症途径的调节作用
Heliyon. 2024 Jan 27;10(3):e24909. doi: 10.1016/j.heliyon.2024.e24909. eCollection 2024 Feb 15.
二萜类化合物来自褐藻及其抗炎活性。
Mar Drugs. 2021 Nov 27;19(12):677. doi: 10.3390/md19120677.
4
Synthesis compound XCR-7a ameliorates LPS-induced inflammatory response by inhibiting the phosphorylation of c-Fos.化合物 XCR-7a 通过抑制 c-Fos 的磷酸化来改善 LPS 诱导的炎症反应。
Biomed Pharmacother. 2022 Jan;145:112468. doi: 10.1016/j.biopha.2021.112468. Epub 2021 Nov 27.
5
Inhibitory effects of sulforaphane on NLRP3 inflammasome activation.萝卜硫素对 NLRP3 炎性小体激活的抑制作用。
Mol Immunol. 2021 Dec;140:175-185. doi: 10.1016/j.molimm.2021.10.014. Epub 2021 Oct 28.
6
Ergosta-7,9(11),22-trien-3β-ol Attenuates Inflammatory Responses via Inhibiting MAPK/AP-1 Induced IL-6/JAK/STAT Pathways and Activating Nrf2/HO-1 Signaling in LPS-Stimulated Macrophage-like Cells.麦角甾-7,9(11),22-三烯-3β-醇通过抑制丝裂原活化蛋白激酶/活化蛋白-1诱导的白细胞介素-6/ Janus激酶/信号转导和转录激活因子途径以及激活脂多糖刺激的巨噬细胞样细胞中的核因子E2相关因子2/血红素加氧酶-1信号来减轻炎症反应。
Antioxidants (Basel). 2021 Sep 8;10(9):1430. doi: 10.3390/antiox10091430.
7
Functional Interplay between Methyltransferases and Inflammasomes in Inflammatory Responses and Diseases.甲基转移酶与炎症小体在炎症反应和疾病中的功能相互作用。
Int J Mol Sci. 2021 Jul 15;22(14):7580. doi: 10.3390/ijms22147580.
8
Sulforaphane: A review of its therapeutic potentials, advances in its nanodelivery, recent patents, and clinical trials.莱菔硫烷：治疗潜力的综述、纳米递药的进展、最新专利和临床试验。
Phytother Res. 2021 Oct;35(10):5440-5458. doi: 10.1002/ptr.7176. Epub 2021 Jun 28.
9
Sulforaphane inhibits NLRP3 inflammasome activation in microglia through Nrf2-mediated miRNA alteration.萝卜硫素通过Nrf2介导的miRNA改变抑制小胶质细胞中NLRP3炎性小体的激活。
Immunol Lett. 2021 May;233:20-30. doi: 10.1016/j.imlet.2021.03.004. Epub 2021 Mar 9.
10
Examination of Novel Immunomodulatory Effects of L-Sulforaphane.L-萝卜硫素的新型免疫调节作用研究
Nutrients. 2021 Feb 12;13(2):602. doi: 10.3390/nu13020602.