Kong Lingnan, Xu Famei, Yao Yukuan, Gao Zhihui, Tian Peng, Zhuang Shichao, Wu Di, Li Tangyue, Cai Yanling, Li Jing
Affiliated Hospital of Weifang Medical University, School of Clinical Medicine, Weifang Medical University, Weifang, China.
Department of Pathology, Zibo Central Hospital, Zibo, China.
Front Oncol. 2023 Jul 3;13:1142755. doi: 10.3389/fonc.2023.1142755. eCollection 2023.
Ovarian cancer (OVCA) is one of the most prevalent malignant tumors of the female reproductive system, and its diagnosis is typically accompanied by the production of ascites. Although liquid biopsy has been widely implemented recently, the diagnosis or prognosis of OVCA based on liquid biopsy remains the primary emphasis.
In this study, using proximity barcoding assay, a technique for analyzing the surface proteins on single extracellular vesicles (EVs). For validation, serum and ascites samples from patients with epithelial ovarian cancer (EOC) were collected, and their levels of CDCP1 was determined by enzyme-linked immunosorbent assay. Tissue chips were prepared to analyze the relationship between different expression levels of CDCP1 and the prognosis of ovarian cancer patients.
We discovered that the CUB domain-containing protein 1+ (CDCP1+) EVs subcluster was higher in the ascites of OVCA patients compared to benign ascites. At the same time, the level of CDCP1 was considerably elevated in the ascites of OVCA patients. The overall survival and disease-free survival of the group with high CDCP1 expression in EOC were significantly lower than those of the group with low expression. In addition, the receiver operating characteristic curve demonstrates that EVs-derived CDCP1 was a biomarker of early response in OVCA ascites.
Our findings identified a CDCP1+ EVs subcluster in the ascites of OVCA patients as a possible biomarker for EOC prevention.
卵巢癌（OVCA）是女性生殖系统中最常见的恶性肿瘤之一，其诊断通常伴随着腹水的产生。尽管液体活检最近已广泛应用，但基于液体活检的卵巢癌诊断或预后仍然是主要重点。
在本研究中，使用邻近条形码分析技术，该技术用于分析单个细胞外囊泡（EVs）上的表面蛋白。为了进行验证，收集了上皮性卵巢癌（EOC）患者的血清和腹水样本，并通过酶联免疫吸附测定法测定其CDCP1水平。制备组织芯片以分析CDCP1不同表达水平与卵巢癌患者预后之间的关系。
我们发现，与良性腹水相比，OVCA患者腹水中含CUB结构域蛋白1阳性（CDCP1+）的EVs亚群更高。同时，OVCA患者腹水中CDCP1水平显著升高。EOC中CDCP1高表达组的总生存期和无病生存期明显低于低表达组。此外，受试者工作特征曲线表明，EVs衍生的CDCP1是OVCA腹水中早期反应的生物标志物。
我们的研究结果确定了OVCA患者腹水中的CDCP1+ EVs亚群可能是EOC预防的生物标志物。
