Wikantyasning Erindyah Retno, Da'i Muhammad, Cholisoh Zakky, Kalsum Ummi
Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Jl. A. Yani 157, Sukoharjo, Indonesia.
Study Program of Pharmacy, STIKES Telogorejo, Jl. Yos Sudarso, Semarang, Jawa Tengah, Indonesia.
J Pharm Bioallied Sci. 2023 Apr-Jun;15(2):81-87. doi: 10.4103/jpbs.jpbs_646_22. Epub 2023 Jun 8.
Because of their sensitive and selective responses to a wide variety of analytes, colorimetric sensors have gained widespread acceptance in recent years. Gold nanoparticles (AuNPs) are widely employed in visual sensor strategies due to their high stability and ease of use. Combining AuNPs with a responsive polymer can result in distinct surface plasmon resonance (SPR) changes that can be utilized as colorimetric biosensors.
The purpose of this research is to develop a colorimetric-based sensor through the utilization of the optical properties of gold nanoparticles (AuNPs) crosslinked with pH-responsive polymers poly (acrylic acid) (PAA) conjugated to 3-aminophenyl boronic acid (APBA).
The polymer (PAA) was synthesized via RAFT polymerization. The inversed Turkevic method was used to produce AuNPs, which were subsequently used in a self-assembly process using poly (acrylic acid)-aminophenyl boronic acid (PAA-APBA) to create the self-assembled AuNPs-APBA-PAA. The particle size, zeta potential, and reversibility of the polymer-modified gold nanoparticles were determined using a transmission electron microscope (TEM), a particle size analyzer (PSA), and an Ultraviolet-Visible spectrophotometer (UV-Vis spectrophotometer). Visual, UV-Vis spectrophotometer and TEM observations confirmed the system's ability to identify bacteria. Statistical analysis was performed using a one-way analysis of variance using Excel software.
Using UV-Vis spectrophotometry, the particle size of AuNPs was determined to be 25.7 nm, and the maximum absorbance occurred at 530 nm. AuNPs PAA APBA colloid exhibited an absorbance maximum of 532 nm, a zeta potential of -41.53, and a pH transition point between 4 and 5. At concentrations of 4.5 x 10 CFU/mL, the color of the system sensors changed from red to blue after 15 hours of incubation, whereas at concentrations of 1.2 x 10 CFU/mL, the color changed to purple immediately after mixing. The TEM confirmed that the detection mechanism is based on the boronate-polyol bonding of saccharides on the outer membranes of and .
The use of APBA in conjunction with pH-responsive PAA polymers containing AuNPs to detect and bacteria induces a maximum wavelength transition, followed by a color change from red to blue. By the process of de-swelling of the responsive polymer, which induces the aggregation of the AuNPs, the established sensor system is able to alter the color. The conjugated polymer and gold nanoparticle-based sensor system demonstrated a promising method for bacterial detection.
由于比色传感器对多种分析物具有灵敏且选择性的响应，近年来已获得广泛认可。金纳米颗粒（AuNPs）因其高稳定性和易用性而被广泛应用于视觉传感器策略中。将AuNPs与响应性聚合物相结合可导致明显的表面等离子体共振（SPR）变化，这可被用作比色生物传感器。
本研究的目的是通过利用与pH响应性聚合物聚（丙烯酸）（PAA）交联并与3-氨基苯硼酸（APBA）共轭的金纳米颗粒（AuNPs）的光学特性来开发一种基于比色的传感器。
通过可逆加成-断裂链转移（RAFT）聚合合成聚合物（PAA）。采用逆Turkevic法制备AuNPs，随后将其用于使用聚（丙烯酸）-氨基苯硼酸（PAA-APBA）的自组装过程中，以创建自组装的AuNPs-APBA-PAA。使用透射电子显微镜（TEM）、粒度分析仪（PSA）和紫外可见分光光度计（UV-Vis分光光度计）测定聚合物修饰的金纳米颗粒的粒径、zeta电位和可逆性。视觉、UV-Vis分光光度计和TEM观察证实了该系统识别细菌的能力。使用Excel软件进行单因素方差分析进行统计分析。
使用UV-Vis分光光度法测定AuNPs的粒径为25.7nm，最大吸光度出现在530nm处。AuNPs PAA APBA胶体的最大吸光度为532nm，zeta电位为-41.53，pH转变点在4和5之间。在浓度为4.5×10CFU/mL时，系统传感器的颜色在孵育15小时后从红色变为蓝色，而在浓度为1.2×10CFU/mL时，混合后颜色立即变为紫色。TEM证实检测机制基于细菌外膜上糖类的硼酸酯-多元醇键合。
将APBA与含有AuNPs的pH响应性PAA聚合物结合使用以检测细菌会引起最大波长转变，随后颜色从红色变为蓝色。通过响应性聚合物的去溶胀过程，其诱导AuNPs的聚集，所建立的传感器系统能够改变颜色。基于共轭聚合物和金纳米颗粒的传感器系统展示了一种有前景的细菌检测方法。
