College of Materials Science and Engineering, Hebei University of Engineering, 19 Taiji Road, Handan 056038, China.
School of Environmental and Municipal Engineering, Qingdao University of Technology, 11 Fushun Road, Qingdao 266033, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Nov 15;261:120004. doi: 10.1016/j.saa.2021.120004. Epub 2021 May 25.
Iohexol (IHO), as one of iodinated X-ray contrast, is often used as not only a chemical marker for tracking wastewater contamination in aquatic environment, but also an ideal glomerular filtration rate marker for explorating kidney disease. To these aims, it is important to establish reliable, fast, and cheap methods to detect IHO in environmental and biological samples. This work describes for the first time the development of a selective, sensitive and reliable colorimetric sensing assay for the fast determination of IHO in environmental and biological samples based on 3-aminophenylboronic acid (3-APBA) mediated aggregation of gold nanoparticles (AuNPs). In this approach, 3-APBA can assemble on the AuNPs surface through electrostatic interaction between its amino groups with the negatively charged citrate stabilizer of AuNPs to form AuNP@3-APBA. Subsequently, the aggregation and visual color change of the assembled AuNP@3-APBA are induced by the covalent reaction between boronic acid ligands of 3-APBA and cis-diols of IHO. The developed assay presented a very simple operating procedure and a rapid analysis time of around 10 min. The developed assay also exhibited good selectivity and a low limit of detection (LOD) of 0.005 mM for detecting IHO. Moreover, the developed assay showed comparable accuracy and precision to the high-performance liquid chromatography-diode array detector (HPLC-DAD) method when used for the rapid determination of IHO in river water and human urine samples. The recoveries of IHO at three spiking levels were in the range of 91.5-106.3% with relative standard deviation (RSD) values below 6.39%.
碘海醇（IHO）作为一种碘 X 射线造影剂，不仅常被用作示踪水污染的化学标记物，还常被用作研究肾病的理想肾小球滤过率标记物。为实现这些目标，建立可靠、快速且廉价的方法来检测环境和生物样本中的 IHO 非常重要。本工作首次描述了一种基于 3-氨苯基硼酸（3-APBA）介导的金纳米粒子（AuNPs）聚集的选择性、灵敏和可靠的比色传感测定法，用于快速测定环境和生物样本中的 IHO。在该方法中，3-APBA 可以通过其氨基与 AuNPs 表面带负电荷的柠檬酸稳定剂之间的静电相互作用组装在 AuNPs 表面上，形成 AuNP@3-APBA。随后，组装的 AuNP@3-APBA 的聚集和可见颜色变化是由 3-APBA 的硼酸配体与 IHO 的顺二醇之间的共价反应引起的。所开发的测定法具有非常简单的操作程序和大约 10 分钟的快速分析时间。该测定法还表现出良好的选择性和 0.005 mM 的低检测限（LOD），用于检测 IHO。此外，当用于快速测定河水和人尿样本中的 IHO 时，所开发的测定法与高效液相色谱-二极管阵列检测器（HPLC-DAD）法具有相当的准确性和精密度。IHO 在三个加标水平的回收率在 91.5-106.3%范围内，相对标准偏差（RSD）值低于 6.39%。
