Institute of Life Sciences & Biomedical Collaborative Innovation Center of Zhejiang Province, Wenzhou University, Wenzhou 325035, China.
College of Veterinary Medicine, Jilin University, Changchun 130062, China.
Life Sci. 2023 Sep 15;329:121956. doi: 10.1016/j.lfs.2023.121956. Epub 2023 Jul 19.
Escherichia coli Nissle 1917 (EcN) has become a research hotspot in inflammatory bowel disease (IBD). It has a strong targeting effect on the colon, and has some therapeutic effect on inflammatory bowel disease. EcN is prepared into EcN ghosts, which also retain EcN's biological characteristics. Consequently, EcN ghosts are used for drug delivery. This study evaluated the safety and efficacy of EcN ghosts as carriers of drugs for treating IBD in zebrafish. Caco-2 cell adhesion experiments and zebrafish intestinal adhesion experiments demonstrated that EcN ghosts was highly adherent to the intestine. Additionally, oral administration of EcN ghosts attenuated dextran sulfate sodium-induced IBD symptoms by inhibiting neutrophil chemotaxis and reactive oxygen species production in larval zebrafish. Because of the unique biological functions of EcN ghosts, it may serve as a strategy for future targeted drug delivery in IBD treatment.
大肠杆菌 Nissle 1917（EcN）已成为炎症性肠病（IBD）的研究热点。它对结肠具有很强的靶向作用，对炎症性肠病具有一定的治疗作用。EcN 被制成 EcN 鬼，它还保留了 EcN 的生物学特性。因此，EcN 鬼被用于药物递送。本研究评估了 EcN 鬼作为治疗 IBD 的药物载体在斑马鱼中的安全性和疗效。Caco-2 细胞黏附实验和斑马鱼肠道黏附实验表明，EcN 鬼对肠道具有高度黏附性。此外，EcN 鬼的口服给药通过抑制幼虫斑马鱼中性粒细胞趋化性和活性氧的产生，减轻了葡聚糖硫酸钠诱导的 IBD 症状。由于 EcN 鬼具有独特的生物学功能，它可能成为未来 IBD 治疗中靶向药物递送的一种策略。
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