School of Stomatology, Jinan University, Guangzhou 510632, China.
Hospital of Stomatology, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2023 Aug 1;41(4):395-404. doi: 10.7518/hxkq.2023.2023055.
This study aims to investigate the effects and mechanisms of chondroitin sulfate (CS), dermatan sulfate (DS), and heparin (HEP) on chondrogenesis of murine chondrogenic cell line (ATDC5) cells and the maintenance of murine articular cartilage .
ATDC5 and articular cartilage tissue explant were cultured in the medium containing different sulfated glycosaminoglycans. Cell proliferation, differentiation, cartilage formation, and mechanism were observed using cell proliferation assay, Alcian blue staining, real-time quantitative polymerase chain reaction (RT-qPCR), and Western blot, respectively.
Results showed that HEP and DS primarily activated the bone morphogenetic protein (BMP) signal pathway, while CS primarily activated the protein kinase B (AKT) signal pathway, further promoted ATDC5 cell proliferation and matrix production, and increased Sox9, Col2a1, and Aggrecan expression.
This study investigated the differences and mechanisms of different sulfated glycosaminoglycans in chondrogenesis and cartilage homeostasis maintenance. HEP promotes cartilage formation and maintains the normal state of cartilage tissue , while CS plays a more effective role in the regeneration of damaged cartilage tissue.
本研究旨在探讨硫酸软骨素（CS）、硫酸皮肤素（DS）和肝素（HEP）对鼠软骨细胞系（ATDC5）细胞软骨生成和维持鼠关节软骨的作用和机制。
在含有不同硫酸化糖胺聚糖的培养基中培养 ATDC5 和关节软骨组织外植体。通过细胞增殖试验、阿利新蓝染色、实时定量聚合酶链反应（RT-qPCR）和 Western blot 分别观察细胞增殖、分化、软骨形成和机制。
结果表明，HEP 和 DS 主要激活骨形态发生蛋白（BMP）信号通路，而 CS 主要激活蛋白激酶 B（AKT）信号通路，进一步促进 ATDC5 细胞增殖和基质生成，并增加 Sox9、Col2a1 和 Aggrecan 的表达。
本研究探讨了不同硫酸化糖胺聚糖在软骨生成和软骨稳态维持中的差异和机制。HEP 促进软骨形成并维持软骨组织的正常状态，而 CS 在受损软骨组织的再生中发挥更有效的作用。
