Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
Reprod Domest Anim. 2023 Sep;58(9):1298-1310. doi: 10.1111/rda.14434. Epub 2023 Jul 20.
The comparison between adding egg yolks (EY) of chicken, duck, goose, pigeon, Japanese quail or turkey to the Tris glycerol extender on the quality of ram semen before freezing and post-thawing was evaluated. The correlation between fatty acids levels in egg yolks of different avian species and the post-thawed quality of ram semen was studied. The pooled ejaculates collected from five rams were extended with Tris EY glycerol extenders containing the EY of chicken, duck, goose, pigeon, quail or turkey and cryopreserved at -196°C. The straws were evaluated before freezing and post-thawing for sperm motility using a sperm cell analyser, vitality using a FluoVit kit and abnormality using a SpermBlue stain besides plasma-membrane and DNA integrities using a hypo-osmotic swelling test and a Halomax kit, respectively. The moisture, ash, protein and fatty acid (FA) contents of EY of chicken, duck, goose, pigeon, quail and turkey were analysed using a gas chromatograph. The chicken and quail EY extenders significantly improved the total progressive motility (32.05 ± 1.41 and 31.68 ± 1.43, respectively), vitality, plasma membrane and DNA integrities and abnormalities of post-thawing ram semen in comparison with other EY extenders. Pigeon EY had the lowest saturated fatty acids (SFAs) in comparison with the other types of EYs. The chicken and turkey EYs had the lowest percentage of (monounsaturated fatty acids) MUFAs in comparison with the other types of EYs. The highest percentage of polyunsaturated fatty acids (PUFAs) was observed in the turkey, pigeon and chicken EYs which were considered double or triple their percentage in duck and goose EYs, respectively. Significant positive correlations existed between SFAs levels and total motility, vitality, plasma membrane functionality and DNA integrity (0.77, 0.80, 0.67, 0.52, respectively). Significant negative correlations existed between gondoic EY levels and total motility, vitality, plasma membrane functionality and DNA integrity. In conclusion, the EYs of duck, goose, pigeon or turkey cannot substitute the chicken EY in ram semen extenders as they gave lower post-thawing quality. The quail EY can be used as a good replacer for chicken EY in the extender used for cryopreservation of ram semen. The EY composition of FAs can significantly affect the quality of ram semen post-thawing.
比较了在冷冻和解冻前向 Tris 甘油 extender 中添加鸡、鸭、鹅、鸽、鹌鹑或火鸡的蛋黄（EY）对公羊精液质量的影响。研究了不同禽类蛋黄中的脂肪酸水平与公羊精液解冻后质量之间的相关性。从五头公羊收集混合精液，用含鸡、鸭、鹅、鸽、鹌鹑或火鸡蛋黄的 Tris EY 甘油 extender 进行扩展，然后在-196°C 下冷冻保存。在冷冻和解冻后，使用精子细胞分析仪评估精子活力，使用 FluoVit 试剂盒评估活力，使用 SpermBlue 染色评估异常，使用低渗肿胀试验和 Halomax 试剂盒评估质膜和 DNA 完整性。使用气相色谱仪分析鸡、鸭、鹅、鸽、鹌鹑和火鸡蛋黄的水分、灰分、蛋白质和脂肪酸（FA）含量。与其他蛋黄 extender 相比，鸡和鹌鹑蛋黄 extender 显著提高了解冻后公羊精液的总前向运动性（分别为 32.05±1.41 和 31.68±1.43）、活力、质膜和 DNA 完整性以及异常率。与其他类型的 EYs 相比，鸽子 EY 的饱和脂肪酸（SFAs）含量最低。与其他类型的 EYs 相比，鸡和火鸡 EYs 的单不饱和脂肪酸（MUFAs）百分比最低。在火鸡、鸽子和鸡 EYs 中观察到最高的多不饱和脂肪酸（PUFAs）百分比，分别是鸭和鹅 EYs 的两倍或三倍。SFAs 水平与总运动性、活力、质膜功能和 DNA 完整性呈显著正相关（分别为 0.77、0.80、0.67、0.52）。Gondoic EY 水平与总运动性、活力、质膜功能和 DNA 完整性呈显著负相关。总之，鸭、鹅、鸽或火鸡的 EYs 不能替代公羊精液 extender 中的鸡 EY，因为它们的解冻后质量较低。鹌鹑 EY 可以作为鸡 EY 在公羊精液冷冻保存中的良好替代品。FA 的 EY 组成会显著影响解冻后公羊精液的质量。
