State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, 310058, Hangzhou, China.; Xianghu Laboratory, Hangzhou 311231, China.
Department of Plant Breeding and Genetics, University of Agriculture, 38000 Faisalabad, Pakistan.
J Hazard Mater. 2023 Oct 5;459:132070. doi: 10.1016/j.jhazmat.2023.132070. Epub 2023 Jul 17.
Nano-enabled strategies have emerged as promising alternatives to resolve heavy metals (HMs) related harms in an eco-friendly manner. Here, we explored the potential of biogenic silicon nanoparticles (SiNPs) in alleviating cadmium (Cd) stress in rapeseed (Brassica napus L.) plants by modulating cellular oxidative repair mechanisms. Biogenic SiNPs of spherical shapes with size ranging between 14 nm and 35 nm were synthesized using rice straw extract and characterized through advanced characterization techniques. A greenhouse experiment results showed that SiNPs treatment at 250 mg kg significantly improved growth parameters, including fresh weight (33.3 %) and dry weight (32.6 %) of rapeseed plants than Cd-treated control group. Photosynthesis and leaf gas exchange parameters were also positively influenced by SiNPs treatment, indicating enhanced photosynthetic efficiency. Additionally, SiNPs treatment at 250 mg kg increased the activities of antioxidant enzymes such as superoxide dismutase (19.1 %), peroxidase (33.4 %), catalase (14.4 %), and ascorbate peroxidase (33.8 %), which may play a crucial role in ROS scavenging and reduction in Cd-induced oxidative stress. TEM analysis revealed that SiNPs treatment effectively mitigated Cd-induced damage to leaf ultrastructure, while qPCR analysis showed that SiNPs treatment changed the expressions of the antioxidant defense and stress related genes. Moreover, SiNPs treatment significantly influenced the Cd accumulation and Si contents in plants. Overall, our findings revealed that biogenic SiNPs have great potential to serve as a sustainable, eco-friendly, and non-toxic alternative for the remediation of Cd toxicity in rapeseed plants.
纳米技术已经成为一种很有前途的方法，可以用环保的方式解决重金属（HMs）相关的危害。在这里，我们通过调节细胞氧化修复机制，探索了生物硅纳米颗粒（SiNPs）在缓解油菜（Brassica napus L.）植物镉（Cd）胁迫方面的潜力。使用稻草提取物合成了具有 14nm 至 35nm 之间尺寸的球形生物 SiNPs，并通过先进的表征技术对其进行了表征。温室实验结果表明，与 Cd 处理对照组相比，250mg/kg 的 SiNPs 处理显著提高了油菜的生长参数，包括鲜重（33.3%）和干重（32.6%）。SiNPs 处理还对光合作用和叶片气体交换参数产生了积极影响，表明其提高了光合作用效率。此外，250mg/kg 的 SiNPs 处理增加了超氧化物歧化酶（19.1%）、过氧化物酶（33.4%）、过氧化氢酶（14.4%）和抗坏血酸过氧化物酶（33.8%）等抗氧化酶的活性，这可能在清除活性氧和减轻 Cd 诱导的氧化应激方面发挥了关键作用。TEM 分析表明，SiNPs 处理有效地减轻了 Cd 诱导的叶片超微结构损伤，而 qPCR 分析表明，SiNPs 处理改变了抗氧化防御和应激相关基因的表达。此外，SiNPs 处理显著影响了植物对 Cd 的积累和 Si 的含量。总的来说，我们的研究结果表明，生物 SiNPs 具有作为一种可持续、环保、无毒的油菜植物修复 Cd 毒性的替代方法的巨大潜力。
