Drehval' O A, Chervatiuk N V, Cherevach N V, Vinnikov A I
Dniepropetrovsk National University.
Mikrobiol Z. 2003 May-Jun;65(3):14-20.
Mineral composition of culture medium has been studied for its effect on the processes of growth, spore-and-toxin formation of entomopathogenic bacteria Bacillus thuringiensis. It has been shown that salts Na2SO4, Na2SiO3.9H2O, MgSO4.7H2O increase the yield of biomass and endotoxin; FeSO4.7H2O, CaCl2, MnSO4.4H2O increase only the yield of biomass; CdSO4.7H2O and CoCl2.6H2O--inhibit the processes of spore-and-toxin formation, but increase the cell titre. A complex of mineral salts has been found which takes positive effect on the biomass accumulation, delta-endotoxin synthesis and insecticide activity of the studied bacteria. It is shown that the drug "Gumat" besides other elements of the culture medium completely provides bacteria with necessary mineral compounds.
研究了培养基的矿物质组成对昆虫病原细菌苏云金芽孢杆菌生长、孢子和毒素形成过程的影响。结果表明，Na2SO4、Na2SiO3·9H2O、MgSO4·7H2O等盐类可提高生物量和内毒素产量；FeSO4·7H2O、CaCl2、MnSO4·4H2O仅提高生物量产量；CdSO4·7H2O和CoCl2·6H2O抑制孢子和毒素形成过程，但提高细胞滴度。已发现一种复合矿物盐对所研究细菌的生物量积累、δ-内毒素合成及杀虫活性具有积极作用。结果表明，除培养基的其他成分外，“Gumat”药物能为细菌完全提供所需的矿物质化合物。
