Department of Haematology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
National Clinical Research Centre for Haematologic Diseases, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
J Int Med Res. 2023 Jul;51(7):3000605231187932. doi: 10.1177/03000605231187932.
To investigate the impact of natural killer (NK) cells in the graft on the outcome following haploidentical peripheral blood stem cell transplantation (haplo-PBSCT).
We retrospectively collected data from patients who had undergone haplo-PBSCT at our centre from January 2019 to November 2021. The percentage of NK cells in stem cell grafts was detected by flow cytometry. Based on the median (range) count of NK cells (1.8 [0.4-6.0] × 10/kg), patients were separated into high and low NK cell count groups.
Data from 96 patients were analysed. Patients were evenly distributed (48 in each group) into high and low NK cell count groups. There was no significant difference in neutrophil and platelet recovery between the two groups. However, the rates of febrile neutropenia, bacterial infection, and invasive fungal disease (IFD) were significantly reduced in the high compared with the low NK cell count group. There was no significant difference in rates of cytomegalovirus (CMV) and Epstein-Barr virus (EBV) infections between groups. There was no significant difference between groups in grades II and above acute graft versus host disease (GVHD), progression-free survival (PFS), or overall survival (OS).
A high number of NK cells in the graft may reduce febrile neutropenia, IFD and bacterial infection following haplo-PBSCT but it has no significant effect on aGVHD, PFS, or OS.
研究供体 NK 细胞对单倍体外周血造血干细胞移植（haplo-PBSCT）后结局的影响。
我们回顾性收集了 2019 年 1 月至 2021 年 11 月在我中心接受 haplo-PBSCT 的患者数据。通过流式细胞术检测干细胞移植物中 NK 细胞的百分比。根据 NK 细胞计数的中位数（范围）（1.8[0.4-6.0]×10/kg），将患者分为高 NK 细胞计数组和低 NK 细胞计数组。
分析了 96 例患者的数据。患者在高 NK 细胞计数组和低 NK 细胞计数组之间均匀分布（每组 48 例）。两组间中性粒细胞和血小板恢复无显著差异。然而，高 NK 细胞计数组发热性中性粒细胞减少、细菌感染和侵袭性真菌感染（IFD）的发生率明显低于低 NK 细胞计数组。两组间巨细胞病毒（CMV）和 EBV 感染的发生率无显著差异。两组间 II 级及以上急性移植物抗宿主病（GVHD）、无进展生存率（PFS）或总生存率（OS）无显著差异。
移植物中 NK 细胞数量较高可能会降低单倍体 PBSCT 后发热性中性粒细胞减少、IFD 和细菌感染的发生率，但对 aGVHD、PFS 或 OS 无显著影响。
