Department of Chemistry, University of Turku, Henrikinkatu 2, 20500 Turku, Finland.
Department of Chemistry, University of Turku, Henrikinkatu 2, 20500 Turku, Finland.
J Inorg Biochem. 2023 Oct;247:112331. doi: 10.1016/j.jinorgbio.2023.112331. Epub 2023 Jul 17.
Two oligonucleotide conjugates sharing the same sequence but incorporating a different 5'-terminal organometallic moiety were synthesized, by either direct mercuration in solution or oximation with an organomercury aldehyde on solid support. The potential of these conjugates to serve as new type of artificial ribonucleases was tested with a complementary 2´-O-methyl-RNA target sequence featuring a single cleavable RNA phosphodiester linkage. Both organomercury oligonucleotides greatly outperformed their metal-free counterparts as well as the previously reported small molecule organomercury RNA cleaving agent in catalytic activity, providing an important proof-of-concept. Compared to state-of-the-art metal-dependent artificial ribonucleases, however, the observed activity was modest.
两种寡核苷酸缀合物具有相同的序列，但在 5'-末端含有不同的有机金属部分，它们通过溶液中的直接汞化或在固体支持物上与有机汞醛肟化反应合成。这些缀合物作为新型人工核糖核酸酶的潜力通过具有单个可切割 RNA 磷酸二酯键的互补 2´-O-甲基-RNA 靶序列进行了测试。两种有机汞寡核苷酸在催化活性方面都远远优于其无金属对应物以及之前报道的小分子有机汞 RNA 切割剂，这提供了一个重要的概念验证。然而，与最先进的金属依赖性人工核糖核酸酶相比，观察到的活性适中。
