Pelá Vinicius Taioqui, Gironda Carlos Condarco, Taira Even Akemi, Brito Leonardo, Pieretti Joana Claudio, Seabra Amedea Barozzi, Cardoso Cristiane de Almeida Baldini, Rodrigues Marcela Charantola, Henrique-Silva Flavio, Buzalaf Marília Afonso Rabelo
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, Bauru, SP, 17012-901, Brazil.
Center for Natural and Human Sciences, Federal University of ABC, Santo André, 09210-580, Brazil.
Clin Oral Investig. 2023 Sep;27(9):5559-5568. doi: 10.1007/s00784-023-05175-z. Epub 2023 Jul 22.
This study evaluated the protective capacity of a sugarcane-derived cystatin (CaneCPI-5) in different vehicles (1-solution and 2-chitosan gel) against erosive dentin wear in situ.
In part-1, 15 volunteers participated in a crossover protocol (solutions): Water; Elmex™ and CaneCPI-5. The volunteers wore an appliance with 4 dentin samples for 5 days. These samples were treated with a drop of the solutions for 1 min (4X/d), then the acquired pellicle (AP) was formed and the samples were subjected to erosive challenges (EROSION: citric acid, for 90 s, 4X/day). 2X/day, half of the samples were also abraded for 15 s (ABRASION). In part-2, 16 volunteers participated in a crossover protocol (gel): No gel, Chitosan gel, Chitosan gel + NaF and Chitosan gel + CaneCPI-5. The volunteers also wore an appliance. The samples were treated once/day with the gel or not for 4 min, then the AP was formed and the samples were subjected to erosive and abrasive challenges, as reported in part-1. Dentin wear was measured by profilometry. Data were analyzed by two-way RM-ANOVA and Sidak's tests (p < 0.05).
Part-1: Elmex™ and CaneCPI-5 significantly reduced dentin loss in comparison with Water for the EROSION/ABRASION conditions (p < 0.05). Part-2, all the treated groups significantly reduced the dentin loss in comparison to the No gel. The greatest reduction was found for the gel + CaneCPI-5 group for the EROSION/ABRASION (p < 0.05).
The solution and chitosan gel containing CaneCPI-5 protected against erosive dentin wear in situ.
These different vehicles are probably sufficient for protecting people with high risk of developing erosive dentin wear.
本研究评估了甘蔗来源的半胱氨酸蛋白酶抑制剂（甘蔗CPI-5）在不同载体（1-溶液和2-壳聚糖凝胶）中对原位侵蚀性牙本质磨损的保护能力。
在第一部分中，15名志愿者参与了交叉实验方案（溶液）：水、Elmex™和甘蔗CPI-5。志愿者佩戴带有4个牙本质样本的装置5天。这些样本用一滴溶液处理1分钟（每天4次），然后形成获得性薄膜（AP），并对样本进行侵蚀性挑战（侵蚀：柠檬酸，90秒，每天4次）。每天2次，一半的样本还进行15秒的磨损（磨损）。在第二部分中，16名志愿者参与了交叉实验方案（凝胶）：无凝胶、壳聚糖凝胶、壳聚糖凝胶+氟化钠和壳聚糖凝胶+甘蔗CPI-5。志愿者也佩戴装置。样本每天用凝胶处理一次或不处理4分钟，然后形成AP，并对样本进行如第一部分所述的侵蚀性和磨损性挑战。通过轮廓测定法测量牙本质磨损。数据通过双向重复测量方差分析和Sidak检验进行分析（p<0.05）。
第一部分：在侵蚀/磨损条件下，与水相比，Elmex™和甘蔗CPI-5显著减少了牙本质损失（p<0.05）。第二部分，与无凝胶组相比，所有处理组均显著减少了牙本质损失。在侵蚀/磨损方面，凝胶+甘蔗CPI-5组减少最多（p<0.05）。
含有甘蔗CPI-5的溶液和壳聚糖凝胶可保护原位侵蚀性牙本质磨损。
这些不同的载体可能足以保护有发展为侵蚀性牙本质磨损高风险的人群。
