Department of Biotechnology, National Institute of Technology Warangal, Warangal, 506004, Telangana, India.
Sci Rep. 2023 Jul 22;13(1):11848. doi: 10.1038/s41598-023-38681-x.
The present study investigates the molecular dynamics of Myc in normal precursors and in different stages (I/II/III/IV) of cohorts of renal cancer using two distinct yet complementary approaches: gene expression and gene coexpression. We also analysed the variation of coexpression networks of Myc through the stage-wise progression of renal cancer cohorts. Myc expression is significantly higher in stage I compared to normal tissue but changed inconsistently across stages of renal cancer. We identified that Myc consistently coexpressed with fourteen genes in the KIPAN [Pan-kidney cohort (KICH + KIRC + KIRP)] and eight in the KIRC (Kidney renal clear cell carcinoma) across all stages, providing potential prognostic and diagnostic biomarkers. Coexpression network complexity decreased from normal precursor tissues to associated tumour stage I in KIPAN and KIRC but was inconsistent after that. In the process of cancer development, there is generally lower cross-tissue cancer network homology observed among coexpressed genes with Myc during the normal to the stage I compared to the stage-wise progression of cancer. Overall, this research provides novel perceptions of the molecular causes of kidney cancer. It also highlights potential genes and pathways crucial for diagnosing and treating this disease.
基因表达和基因共表达，研究了 Myc 在正常前体和不同阶段（I/II/III/IV）的肾癌细胞中的分子动力学。我们还通过肾癌细胞的阶段性进展分析了 Myc 共表达网络的变化。与正常组织相比，Myc 在 I 期的表达明显更高，但在肾细胞癌的不同阶段变化不一致。我们确定 Myc 在 KIPAN（泛肾队列[KICH + KIRC + KIRP]）的所有阶段都与十四个基因一致共表达，在 KIRC（肾透明细胞癌）中则与八个基因一致共表达，为潜在的预后和诊断生物标志物提供了依据。在 KIPAN 和 KIRC 中，从正常前体组织到相关肿瘤 I 期，共表达网络的复杂性降低，但此后并不一致。在癌症发展过程中，与 Myc 共表达的基因在正常到 I 期的跨组织癌症网络同源性通常低于癌症的阶段性进展。总的来说，这项研究为肾癌的分子病因提供了新的认识。它还突出了诊断和治疗这种疾病的关键基因和途径。
