Department of Biology, Jeju National University, Jeju 63243, Republic of Korea.
Center for Life Science (HCLS), Harbin Institute of Technology, No.92 West Dazhi Street, Nangang District, Harbin City, Hei Longjiang Province, P.R. China.
J Microbiol Biotechnol. 2023 Nov 28;33(11):1495-1505. doi: 10.4014/jmb.2306.06040. Epub 2023 Jul 24.
The western honeybee L., a vital crop pollinator and producer of honey and royal jelly, faces numerous threats including diseases, chemicals, and mite infestations, causing widespread concern. While extensive research has explored the link between gut microbiota and their hosts. However, the impact of infestation remains understudied. In this study, we employed massive parallel amplicon sequencing assays to examine the diversity and structure of gut microbial communities in adult bee groups, comparing healthy (NG) and -infested (VG) samples. Additionally, we analyzed -infested hives to assess the whole body of larvae. Our results indicated a notable prevalence of the genus in larvae and the genera , unidentified , and in adult bees. However, no statistically significant difference was observed between NG and VG. Furthermore, our PICRUSt analysis demonstrated distinct KEGG classification patterns between larval and adult bee groups, with larvae displaying a higher abundance of genes involved in cofactor and vitamin production. Notably, despite the complex nature of the honeybee bacterial community, methanogens were found to be present in low abundance in the honeybee microbiota.
西方蜜蜂 L. 是一种重要的作物传粉媒介，也是蜂蜜和蜂王浆的生产者，但它面临着包括疾病、化学物质和螨虫侵袭在内的多种威胁，这引起了广泛关注。虽然有大量研究探讨了肠道微生物群与其宿主之间的联系，但螨虫侵袭的影响仍未得到充分研究。在这项研究中，我们采用大规模平行扩增子测序分析，比较了健康（NG）和螨虫侵袭（VG）样本的成年蜜蜂群体的肠道微生物群落多样性和结构。此外，我们还分析了螨虫侵袭的蜂群，以评估幼虫的整个身体。我们的结果表明，幼虫中属 的流行率很高，而属 、未鉴定属 和 在成年蜜蜂中流行。然而，NG 和 VG 之间没有观察到统计学上的显著差异。此外，我们的 PICRUSt 分析表明，幼虫和成年蜜蜂群体之间存在明显不同的 KEGG 分类模式，幼虫中与辅助因子和维生素生产相关的基因丰度更高。值得注意的是，尽管蜜蜂细菌群落的性质复杂，但在蜜蜂微生物群中发现产甲烷菌的丰度较低。
