Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Gregor-Mendel-Straße 4, 85354 Freising, Germany.
Int J Syst Evol Microbiol. 2021 Feb;71(2). doi: 10.1099/ijsem.0.004633.
As part of a study investigating the microbiome of bee hives and honey, two novel strains (TMW 2.1880 and TMW 2.1889) of acetic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they cannot be assigned to known species. The isolates are Gram-stain-negative, aerobic, pellicle-forming, catalase-positive and oxidase-negative. Cells of TMW 2.1880 are non-motile, thin/short rods, and cells of TMW 2.1889 are motile and occur as rods and long filaments. Morphological, physiological and phylogenetic analyses revealed a distinct lineage within the genus . Strain TMW 2.1880 is most closely related to the type strain of with a 16S rRNA gene sequence similarity of 99.5 %, and ANIb and DDH values of 94.16 and 56.3 %, respectively. The genome of TMW 2.1880 has a size of 1.98 Mb and a G+C content of 55.3 mol%. Strain TMW 2.1889 is most closely related to the type strain of with a 16S rRNA gene sequence similarity of 99.5 %, and ANIb and DDH values of 85.12 and 29.5 %, respectively. The genome of TMW 2.1889 has a size of 2.07 Mb and a G+C content of 60.4 mol%. Ubiquinone analysis revealed that both strains contained Q-10 as the main respiratory quinone. Major fatty acids for both strains were C, C cyclo 8 and summed feature 8, respectively, and additionally C 2-OH only for TMW 2.1880 and C only for TMW 2.1889. Based on polyphasic evidence, the two isolates from honeycombs of represent two novel species of the genus , for which the names sp. nov and sp. nov. are proposed. The designated respective type strains are TMW 2.1880 (=LMG 31882=CECT 30114) and TMW 2.1889 (=LMG 31883=CECT 30113).
在一项研究蜜蜂蜂巢和蜂蜜微生物组的研究中，分离出了两种新型的醋酸菌（TMW 2.1880 和 TMW 2.1889），并通过多相分类学方法对其进行了分类学特征描述，结果表明它们不能被归为已知的物种。这些分离物是革兰氏阴性、需氧、形成菌膜、过氧化氢酶阳性和氧化酶阴性。TMW 2.1880 的细胞是非运动的、细/短杆状，而 TMW 2.1889 的细胞是运动的，呈杆状和长丝状。形态学、生理学和系统发育分析揭示了属内的一个独特谱系。TMW 2.1880 与模式菌株 最为密切相关，16S rRNA 基因序列相似度为 99.5%，ANIb 和 DDH 值分别为 94.16%和 56.3%。TMW 2.1880 的基因组大小为 1.98 Mb，GC 含量为 55.3 mol%。TMW 2.1889 与模式菌株 最为密切相关，16S rRNA 基因序列相似度为 99.5%，ANIb 和 DDH 值分别为 85.12%和 29.5%。TMW 2.1889 的基因组大小为 2.07 Mb，GC 含量为 60.4 mol%。醌分析表明，两种菌株都含有 Q-10 作为主要的呼吸醌。两种菌株的主要脂肪酸分别为 C 16:0、C 18:1ω7c 和总和特征 8，此外 TMW 2.1880 还含有 C 2-OH，而 TMW 2.1889 仅含有 C 16:0。基于多相证据，从蜂巢中分离出的两种菌株代表了属的两个新种，分别提议使用 sp. nov 和 sp. nov. 作为名称。指定的相应模式菌株是 TMW 2.1880（=LMG 31882=CECT 30114）和 TMW 2.1889（=LMG 31883=CECT 30113）。
